Vascular development in the vertebrate pancreas
| Autor: | Stephen Fu, Judith Magenheim, Leilani Marty-Santos, Alethia Villasenor, Simon S. Lee, David M. Barry, D. Berfin Azizoglu, Yuval Dor, Ondine Cleaver, Diana C. Chong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Mesenchyme Morphogenesis Neovascularization Physiologic Vascular Remodeling Biology Epithelium Article Veins Mice 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine medicine Animals Pancreas Molecular Biology Body Patterning Plexus Neural tube Gene Expression Regulation Developmental Arteries Cell Biology Anatomy Capillaries Cell biology 030104 developmental biology medicine.anatomical_structure Vertebrates Blood Vessels Female 030217 neurology & neurosurgery Developmental Biology Blood vessel Artery |
| DOI: | 10.17615/edzc-6460 |
| Popis: | The vertebrate pancreas is comprised of a highly branched tubular epithelium, which is intimately associated with an extensive and specialized vasculature. While we know a great deal about basic vascular anatomy of the adult pancreas, as well as islet capillaries, surprisingly little is known about the ontogeny of its blood vessels. Here, we analyze development of the pancreatic vasculature in the mouse embryo. We show that pancreatic epithelial branches intercalate with the fine capillary plexus of the surrounding pancreatic mesenchyme. Endothelial cells (ECs) within this mesenchyme are heterogeneous from the onset of organogenesis. Pancreatic arteries take shape before veins, in a manner analogous to early embryonic vessels. The main central artery forms during mid-gestation, as a result of vessel coalescence and remodeling of a vascular plexus. In addition, we show that vessels in the forming pancreas display a predictable architecture that is dependent on VEGF signaling. Over-expression of VEGF disrupts vascular patterning and arteriovenous differentiation within the developing pancreas. This study constitutes a first-time in-depth cellular and molecular characterization of pancreatic blood vessels, as they coordinately grow along with the pancreatic epithelium. |
| Databáze: | OpenAIRE |
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