Spiroplasma Infection among Ixodid Ticks Exhibits Species Dependence and Suggests a Vertical Pattern of Transmission
| Autor: | Nariaki Nonaka, Kodai Kusakisako, Keita Matsuno, Shohei Ogata, Wessam Mohamed Ahmed Mohamed, Yongjin Qiu, Ken Katakura, Mohamed Abdallah Mohamed Moustafa, Ryo Nakao, May June Thu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Microbiology (medical) animal structures Spiroplasma Zoology Tick 010603 evolutionary biology 01 natural sciences Microbiology Article ticks Haemaphysalis 03 medical and health sciences stomatognathic system Japan Virology parasitic diseases Ixodes lcsh:QH301-705.5 symbionts 030304 developmental biology 0303 health sciences Genetic diversity biology Host (biology) biology.organism_classification rpoB bacterial infections and mycoses lcsh:Biology (General) Horizontal transmission |
| Zdroj: | Microorganisms, Vol 9, Iss 333, p 333 (2021) Microorganisms Volume 9 Issue 2 |
| ISSN: | 2076-2607 |
| Popis: | Members of the genus Spiroplasma are Gram-positive bacteria without cell walls. Some Spiroplasma species can cause disease in arthropods such as bees, whereas others provide their host with resistance to pathogens. Ticks also harbour Spiroplasma, but their role has not been elucidated yet. Here, the infection status and genetic diversity of Spiroplasma in ticks were investigated using samples collected from different geographic regions in Japan. A total of 712 ticks were tested for Spiroplasma infection by PCR targeting 16S rDNA, and Spiroplasma species were genetically characterized based on 16S rDNA, ITS, dnaA, and rpoB gene sequences. A total of 109 samples originating from eight tick species were positive for Spiroplasma infection, with infection rates ranging from 0% to 84% depending on the species. A linear mixed model indicated that tick species was the primary factor associated with Spiroplasma infection. Moreover, certain Spiroplasma alleles that are highly adapted to specific tick species may explain the high infection rates in Ixodes ovatus and Haemaphysalis kitaokai. A comparison of the alleles obtained suggests that horizontal transmission between tick species may not be a frequent event. These findings provide clues to understand the transmission cycle of Spiroplasma species in wild tick populations and their roles in host ticks. |
| Databáze: | OpenAIRE |
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