Identification and functional characterization of NbMLP28, a novel MLP-like protein 28 enhancing Potato virus Y resistance in Nicotiana benthamiana
| Autor: | Lili Shen, Yongliang Li, Haiyan Jia, Ali Kamran, Kaiqiang Hao, Liyun Song, Fenglong Wang, Yuanxia Qin, Wang Jie, Chaoqun Zhang, Yingjie Liu, Jinguang Yang, Bin Li, Fei Han, Yuanhua Wu |
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| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular 0106 biological sciences Microbiology (medical) Protein family Protein Conformation Overexpression Potyvirus Resistance lcsh:QR1-502 Nicotiana benthamiana NbMLP28 Cyclopentanes Plant disease resistance Plant Roots 01 natural sciences Microbiology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound VIGS Gene Expression Regulation Plant Tobacco Gene silencing Tissue Distribution Oxylipins Potato virus Y Gene Disease Resistance Plant Diseases Plant Proteins 030304 developmental biology Cell Nucleus Genetics Jasmonic acid 0303 health sciences biology fungi food and beverages biology.organism_classification N. Benthamian chemistry MLP-like proteins Signal Transduction Research Article 010606 plant biology & botany |
| Zdroj: | BMC Microbiology, Vol 20, Iss 1, Pp 1-14 (2020) BMC Microbiology |
| Popis: | Background Major latex proteins (MLPs) belong to the MLP subfamily in Bet v 1 protein family and respond to both biotic and abiotic stresses, which play critical roles in plant disease resistance. As the type species of widely distributed and economically devastating Potyvirus, Potato virus Y (PVY) is one of the major constraints to important crop plants including tobacco (Nicotiana benthamiana) worldwide. Despite the great losses owing to PVY infection in tobacco, there is no previous study investigating the potential role of MLPs in developing resistance to viral infection. Results In this study, for the first time we have identified and functionally analyzed the MLP-like protein 28 from N. benthamiana, denoted as NbMLP28 and investigated its role in conferring resistance to N. benthamiana against PVY infection. NbMLP28 was localized to the plasmalemma and nucleus, with the highest level in the root. NbMLP28 gene was hypothesized to be triggered by PVY infection and was highly expressed in jasmonic acid (JA) signaling pathway. Further validation was achieved through silencing of NbMLP28 through virus-induced gene silencing (VIGS) that rendered N. benthamiana plants more vulnerable to PVY infection, contrary to overexpression that enhanced resistance. Conclusions Taken together, this is the first study describing the role of NbMLP28 in tobacco against PVY infection and provide a pivotal point towards obtaining pathogen-resistant tobacco varieties through constructing new candidate genes of MLP subfamily. |
| Databáze: | OpenAIRE |
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