Patterns of Alpha-1-Adrenergic Receptor Expression in Regenerating and Neoplastic Hepatic Tissue
| Autor: | Chi-Ru Lee, Marie C. DeFrances, GeorgeK. Michalopoulos, Diane P. Kost |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Beta-3 adrenergic receptor medicine.medical_specialty Carcinoma Hepatocellular Immunoblotting Gene Expression Alpha (ethology) Biology Pathology and Forensic Medicine Estrogen-related receptor alpha Liver Neoplasms Experimental Internal medicine Tumor Cells Cultured medicine Animals Hepatectomy Humans RNA Messenger Receptor Molecular Biology Alpha-1 adrenergic receptor Liver receptor homolog-1 Liver Neoplasms DNA DNA Neoplasm Cell Biology General Medicine Receptors Adrenergic alpha Molecular biology Rats Inbred F344 Liver regeneration Liver Regeneration Rats medicine.anatomical_structure Endocrinology Liver Hepatocyte |
| Zdroj: | Pathobiology. 60:303-308 |
| ISSN: | 1423-0291 1015-2008 |
| DOI: | 10.1159/000163740 |
| Popis: | As norepinephrine is a potent hepatocyte comitogen through binding to the alpha 1-adrenergic receptor, we have examined mRNA levels of the alpha 1a- and alpha 1b-adrenergic receptor subtypes in normal and regenerating rat hepatocytes as well as in several different rat hepatoma cell lines. All rat hepatomas examined lacked both alpha 1a- and alpha 1b-receptor message and receptor binding in radioligand binding experiments, suggesting that the growth of dedifferentiated neoplastic rat hepatocytes is not regulated by the alpha 1-adrenergic receptor. Interestingly, unlike the rat hepatomas analyzed, the human hepatocellular carcinoma cell line, HepG2, was positive for both alpha 1a and alpha 1b message at 4.5 kb, yet this cell line lacked receptor binding in radioligand binding assays. While normal and regenerating liver is negative for alpha 1a-receptor expression, it is positive for alpha 1b expression and is characterized by the presence of two bands at approximately 4.0 and 3.2 kb which peaked between 20 and 48 h after partial hepatectomy. A dramatic decrease in message level of the lower band and the continued presence of the upper band between 6 and 12 h after partial hepatectomy, and before the peak in DNA synthesis in regenerating rat liver, may correspond with observed differences in alpha 1-receptor function during liver regeneration. |
| Databáze: | OpenAIRE |
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