Molecular epidemiology of human and animal tuberculosis in Ibadan, Southwestern Nigeria

Autor: Y. Hauk, Gilles Vergnaud, Estelle Hildegard Venter, Simeon Cadmus, Christine Pourcel, Jacques Godfroid, Akinbowale Olajide Jenkins
Přispěvatelé: Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2011
Předmět:
MESH: Mycobacterium bovis
MESH: Sequence Analysis
DNA
Veterinary medicine
MESH: Mycobacterium tuberculosis
Swine
Minisatellite Repeats
MESH: Genotype
MESH: Animals
MESH: Tuberculosis
MESH: Swine
Molecular Epidemiology
0303 health sciences
Mycobacterium bovis
biology
Goats
Zoonosis
General Medicine
Bacterial Typing Techniques
3. Good health
MESH: Cattle
Variable number tandem repeat
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Mycobacterium tuberculosis complex
Tandem Repeat Sequences
MESH: Nigeria
DNA
Bacterial
Tuberculosis
Genotype
Nigeria
Multiple Loci VNTR Analysis
MESH: Goats
Microbiology
MESH: Bacterial Typing Techniques
Mycobacterium tuberculosis
03 medical and health sciences
medicine
Animals
Humans
MESH: Molecular Epidemiology
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
030304 developmental biology
MESH: Humans
General Veterinary
Molecular epidemiology
030306 microbiology
Sequence Analysis
DNA
biology.organism_classification
medicine.disease
MESH: DNA
Bacterial
MESH: Tandem Repeat Sequences
MESH: Minisatellite Repeats
Cattle
Zdroj: Vet Microbiol
Vet Microbiol, 2011, 151 (1-2), pp.139-47. ⟨10.1016/j.vetmic.2011.02.037⟩
ISSN: 0378-1135
Popis: International audience; From 2005 to 2007, Mycobacterium tuberculosis complex (MTC) strains were isolated from cattle, goats and pigs samples collected at the Bodija abattoir and from human samples from tuberculosis patients and livestock traders at the Akinyele cattle market in Ibadan, Southwestern Nigeria. Seventy four isolates obtained from humans (24) and livestock (50) were identified as MTC strains. Thirty two isolates were spoligotyped. Nineteen of these 32 isolates were identified as M. tuberculosis whilst 13 were identified as Mycobacterium bovis. M. bovis was isolated from two humans, whereas M. tuberculosis was isolated from a bovine, a pig and a goat. All the M. bovis isolates identified in this study belonged to the Africa 1 clonal complex. Multiple locus VNTR [variable number of tandem repeats] analysis (MLVA) was carried out on the 74 isolates. Three major clusters were defined. Group A consisted of 24 M. tuberculosis isolates (MLVA genotypes 1-18). One strain was isolated from a bovine and one from a pig. Group B consisted of 49 M. bovis strains (MLVA genotypes 19-48), mainly of cattle origin but also included four goat, nine pig and two human isolates. Group C consisted of a single M. tuberculosis isolate (MLVA genotype 49) obtained from a goat. Spoligotyping and MLVA confirmed it as clustering with the East Africa Indian clade found in humans in Sudan and the Republic of Djibouti. The isolation of three M. tuberculosis strains from livestock raises the question of their epidemiological importance as a source of infection for humans.
Databáze: OpenAIRE
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