Molecular epidemiology of human and animal tuberculosis in Ibadan, Southwestern Nigeria
Autor: | Y. Hauk, Gilles Vergnaud, Estelle Hildegard Venter, Simeon Cadmus, Christine Pourcel, Jacques Godfroid, Akinbowale Olajide Jenkins |
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Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2011 |
Předmět: |
MESH: Mycobacterium bovis
MESH: Sequence Analysis DNA Veterinary medicine MESH: Mycobacterium tuberculosis Swine Minisatellite Repeats MESH: Genotype MESH: Animals MESH: Tuberculosis MESH: Swine Molecular Epidemiology 0303 health sciences Mycobacterium bovis biology Goats Zoonosis General Medicine Bacterial Typing Techniques 3. Good health MESH: Cattle Variable number tandem repeat [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Mycobacterium tuberculosis complex Tandem Repeat Sequences MESH: Nigeria DNA Bacterial Tuberculosis Genotype Nigeria Multiple Loci VNTR Analysis MESH: Goats Microbiology MESH: Bacterial Typing Techniques Mycobacterium tuberculosis 03 medical and health sciences medicine Animals Humans MESH: Molecular Epidemiology [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology 030304 developmental biology MESH: Humans General Veterinary Molecular epidemiology 030306 microbiology Sequence Analysis DNA biology.organism_classification medicine.disease MESH: DNA Bacterial MESH: Tandem Repeat Sequences MESH: Minisatellite Repeats Cattle |
Zdroj: | Vet Microbiol Vet Microbiol, 2011, 151 (1-2), pp.139-47. ⟨10.1016/j.vetmic.2011.02.037⟩ |
ISSN: | 0378-1135 |
Popis: | International audience; From 2005 to 2007, Mycobacterium tuberculosis complex (MTC) strains were isolated from cattle, goats and pigs samples collected at the Bodija abattoir and from human samples from tuberculosis patients and livestock traders at the Akinyele cattle market in Ibadan, Southwestern Nigeria. Seventy four isolates obtained from humans (24) and livestock (50) were identified as MTC strains. Thirty two isolates were spoligotyped. Nineteen of these 32 isolates were identified as M. tuberculosis whilst 13 were identified as Mycobacterium bovis. M. bovis was isolated from two humans, whereas M. tuberculosis was isolated from a bovine, a pig and a goat. All the M. bovis isolates identified in this study belonged to the Africa 1 clonal complex. Multiple locus VNTR [variable number of tandem repeats] analysis (MLVA) was carried out on the 74 isolates. Three major clusters were defined. Group A consisted of 24 M. tuberculosis isolates (MLVA genotypes 1-18). One strain was isolated from a bovine and one from a pig. Group B consisted of 49 M. bovis strains (MLVA genotypes 19-48), mainly of cattle origin but also included four goat, nine pig and two human isolates. Group C consisted of a single M. tuberculosis isolate (MLVA genotype 49) obtained from a goat. Spoligotyping and MLVA confirmed it as clustering with the East Africa Indian clade found in humans in Sudan and the Republic of Djibouti. The isolation of three M. tuberculosis strains from livestock raises the question of their epidemiological importance as a source of infection for humans. |
Databáze: | OpenAIRE |
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