Vitamin C treatment of mouse bone marrow-derived dendritic cells enhanced CD8+ memory T cell production capacity of these cells in vivo
| Autor: | Jae Seung Kang, Hang Rae Kim, Jin Hee Kim, Young Il Hwang, Jun Man Hong, Young Joo Jeong, Wang Jae Lee |
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| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Male Immunology Bone Marrow Cells Ascorbic Acid CD8-Positive T-Lymphocytes Immunotherapy Adoptive Interferon-gamma Interleukin 21 Cell Line Tumor medicine Animals Immunology and Allergy Cytotoxic T cell L-Selectin Antigen-presenting cell Cells Cultured Interleukin-15 Mice Inbred BALB C CD40 biology Follicular dendritic cells Vaccination Dendritic Cells Neoplasms Experimental Vitamins Hematology Flow Cytometry Interleukin-12 Survival Analysis Molecular biology Coculture Techniques Mice Inbred C57BL Hyaluronan Receptors medicine.anatomical_structure biology.protein Interleukin 12 Immunologic Memory Memory T cell CD8 |
| Zdroj: | Immunobiology. 219:554-564 |
| ISSN: | 0171-2985 |
| DOI: | 10.1016/j.imbio.2014.03.006 |
| Popis: | Vitamin C has been found to stimulate dendritic cells (DCs) to secrete more IL-12 and thereby drive naïve CD4(+) T cells to differentiate into Th1 cells. In the present study, we evaluated the effect of these vitamin C-treated DCs on CD8(+) T cell differentiation both in vitro and in vivo. Mouse bone marrow-derived DCs were prepared in the presence of GM-CSF and IL-15. With vitamin C treatment, these DCs, when LPS-stimulated, secreted more IL-12p70 and IL-15 than did untreated DCs. And when co-cultured with T cells, they yielded a higher frequency of IFN-γ(+) CD8(+) T cells. Moreover, we found that administering vitamin C-treated and tumor lysate-loaded DCs into mice yielded a higher frequency of CD44(high) CD62L(low) CD8(+) effector and effector memory T cells, which showed an increased ex vivo killing effect of the tumor cells. These DCs also elicited enhanced protective effects against inoculated tumor cells, most probably by way of the increased cytotoxic T cells, as was revealed by the decreased growth of the inoculated tumor cells in these mice. This ex vivo vitamin C treatment effect on DCs can be considered as a strategy for boosting DC vaccination potency against tumors. |
| Databáze: | OpenAIRE |
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