Heterotopic bone formation derived from multipotent stromal cells is not inhibited in aged mice
| Autor: | Christian Beauséjour, Alain Moreau, Gaël Moquin Beaudry, Josée Dépôt, Basma Benabdallah, Saadallah Bouhanik, Geneviève Despars, Cynthia L. Carbonneau |
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| Rok vydání: | 2013 |
| Předmět: |
Senescence
Cancer Research Aging Stromal cell Immunology Cell- and Tissue-Based Therapy Bone Marrow Cells 03 medical and health sciences Mice 0302 clinical medicine Osteogenesis Conditioned medium Immunology and Allergy Medicine Animals Humans Bone formation Genetics (clinical) 030304 developmental biology Aged 0303 health sciences Transplantation business.industry Multipotent Stem Cells Mesenchymal stem cell Heterotopic bone Cell Differentiation Mesenchymal Stem Cells Cell Biology In vitro Unexpected finding Oncology Culture Media Conditioned Cancer research business 030217 neurology & neurosurgery |
| Zdroj: | Cytotherapy. 16(8) |
| ISSN: | 1477-2566 |
| Popis: | Background aims Decreased bone formation with age is believed to arise, at least in part, because of the influence of the senescent microenvironment. In this context, it is unclear whether multipotent stromal cell (MSC)-based therapies would be effective for the treatment of bone diseases. Methods With the use of a heterotopic bone formation model, we investigated whether MSC-derived osteogenesis is impaired in aged mice compared with young mice. Results We found that bone formation derived from MSCs is not reduced in aged mice. These results are supported by the unexpected finding that conditioned media collected from ionizing radiation–induced senescent MSCs can stimulate mineralization and delay osteoclastogenesis in vitro . Conclusions Overall, our results suggest that impaired bone formation with age is mainly cell-autonomous and provide a rationale for the use of MSC-based therapies for the treatment of bone diseases in the elderly. |
| Databáze: | OpenAIRE |
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