Cationic Covalent Organic Framework as an Ion Exchange Material for Efficient Adsorptive Separation of Biomolecules
| Autor: | Imann Mosleh, Alireza Abbaspourrad, Xianghong Qian, Ranil Wickramasinghe, Robert R. Beitle, Christina Carbrello, Hazim Aljewari, Ahmad Reza Khosropour |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Materials science Ion exchange Biomolecule Ion chromatography Cytochromes c Ionic bonding Serum Albumin Bovine Chemical Fractionation Ion Exchange Adsorption Isoelectric point Chemical engineering chemistry Covalent bond Animals Cattle Muramidase General Materials Science Amino Acids Porosity Metal-Organic Frameworks Covalent organic framework |
| Zdroj: | ACS Applied Materials & Interfaces. 13:35019-35025 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.1c11270 |
| Popis: | Although covalent organic frameworks (COFs) have earned significant interest in separation applications, the use of COFs in biomolecule separation remains unexplored. We examined the ionic COF Py-BPy2+-COF as an ion exchange material for biomolecule separation. After characterizing the properties of the synthesized COF with a variety of techniques, binding experiments with both large and small biomolecules were performed. High adsorption capacities of amino acids with different hydrophobicity and charge, as well as proteins of different isoelectric points and molecular weights, were determined in batch equilibrium experiments. Desorption experiments with mixtures of model proteins demonstrated an ability to successfully separate one protein from another with the selectivity hypothesized to be a combination of the isoelectric point, hydrophobicity, and ability to penetrate the crystalline material. Overall, the results demonstrated that Py-BPy2+-COF can be exploited as a robust crystalline anion exchange biomolecule separation material. |
| Databáze: | OpenAIRE |
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