1,25-Dihydroxyvitamin D3-glycoside: Identification of a calcinogenic principle of solanum malacoxylon
| Autor: | Kristine M. Bursac, Toni A. McCain, Meinrad Peterlik, M. R. Hughes, Mark R. Haussler, Robert H. Wasserman |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Vitamin
chemistry.chemical_classification Chromatography Chloroform biology Hydroxycholecalciferols Chemistry Stereochemistry Glycoside General Medicine Plants biology.organism_classification Mass Spectrometry General Biochemistry Genetics and Molecular Biology Sterol chemistry.chemical_compound Hydrolysis Sephadex Dihydroxycholecalciferols Spectrophotometry Ultraviolet Glycosides Silicic acid General Pharmacology Toxicology and Pharmaceutics Solanum glaucophyllum |
| Zdroj: | Life Sciences. 18:1049-1056 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(76)90137-5 |
| Popis: | The water soluble calcinogenic factor present in the plant Solanum malacoxylon is partially purified by selective extraction and chromatography on silicic acid and then hydrolyzed with a mixed preparation of glycosidases from the sea worm, Charonia lampus . Hydrolysis produces a chloroform soluble factor with biologic characteristics of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the hormonal form of vitamin D. Purification of this factor is accomplished by chromatography on Sephadex LH-20, silicic acid, and Celite columns, yielding 3 μg of active material. During the isolation, bioactivity (as assessed by the ability of fractions to compete with labeled 1,25-(OH)2D3 for binding to a specific intestinal receptor protein) migrates exactly with authentic tritiated 1,25-(OH)2D3. The purified factor has an ultraviolet absorption spectrum identical to that of 1,25-(OH)2D3 and analysis via direct probe mass spectrometry yields a parent molecular ion of m/e 416 and a fragmentation pattern indistinguishable from synthetic 1,25-(OH)2D3 hormone. We therefore conclude that the vitamin D-like principle in Solanum malacoxylon is a sterol-glycoside which contains the 1,25-(OH)2D3 molecule as its active sterol component. |
| Databáze: | OpenAIRE |
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