An in vitro assay to quantify satellite cell activation using isolated mouse myofibers
| Autor: | W.W.M. Pim Pijnappel, Emma Boertjes, Gerben J. Schaaf, Stela Bozhilova, Rodrigo Canibano-Fraile |
|---|---|
| Přispěvatelé: | Pediatrics, Erasmus MC other, Clinical Genetics |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Science (General)
Satellite Cells Skeletal Muscle Cytological Techniques Muscle Fibers Skeletal Cell General Biochemistry Genetics and Molecular Biology Q1-390 Mice Protocol medicine Animals Myocyte In vitro study Microscopy General Immunology and Microbiology biology Stem Cells General Neuroscience Cell based assays biology.organism_classification In vitro Cell biology medicine.anatomical_structure Cell isolation Satellite (biology) Cell-based Assays Stem cell Cell activation |
| Zdroj: | STAR Protocols, 2(2):100482. Cell Press STAR Protocols STAR Protocols, Vol 2, Iss 2, Pp 100482-(2021) |
| ISSN: | 2666-1667 |
| Popis: | Summary Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation. Graphical Abstract Highlights • An in vitro assay to study satellite cell activation • An optimized protocol for myofiber isolation • Protocol enables comparison of satellite cell dynamics across different disease models • Versatile 96-well format allows studying of multiple experimental conditions in parallel Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation. |
| Databáze: | OpenAIRE |
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