Tolerogenic dendritic cells are efficiently generated using minocycline and dexamethasone
| Autor: | Jae-Hee Lee, Ji-Wan Kim, Sundong Jang, Kyungjae Kim, Sukgil Song, Sanghyeon Kim, Chan-Su Park, Chong-Kil Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Anti-Inflammatory Agents lcsh:Medicine Bone Marrow Cells Minocycline Pharmacology Article Dexamethasone Autoimmune Diseases Cell therapy 03 medical and health sciences medicine Immune Tolerance Animals lcsh:Science Cells Cultured Autoimmune encephalitis Mice Inbred BALB C Multidisciplinary business.industry lcsh:R Interleukin Granulocyte-Macrophage Colony-Stimulating Factor Cell Differentiation Dendritic Cells In vitro Anti-Bacterial Agents Transplantation Mice Inbred C57BL 030104 developmental biology lcsh:Q Female Immunotherapy Interleukin-4 business Ex vivo medicine.drug |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) SCIENTIFIC REPORTS(7) |
| ISSN: | 2045-2322 |
| Popis: | Tolerogenic dendritic cells (tDCs) represent a promising tool for cellular therapy against autoimmune diseases, allergies, and transplantation rejection. Numerous pharmacological agents are known to induce tDC generation. Minocycline, which has long been used as a broad-spectrum antibiotic, was recently shown to significantly increase the generation of DCs with regulatory properties. Here, we examined the effect of the combination of minocycline with dexamethasone, rapamycin, vitamin D3, and interleukin (IL)-10, which are all known inducers of tDC generation. The highest number of tDCs was generated when minocycline and dexamethasone were used together with granulocyte colony-stimulating factor (GM-SCF) and IL-4. The tolerogenicity of the minocycline/dexamethasone-conditioned tDCs was much better than or at least equal to those of the tDCs generated with either one of these agents, as assessed through in vitro phenotypic and functional assays. In addition, pretreatment with MOG35-55 peptide-pulsed minocycline/dexamethasone-conditioned tDCs significantly ameliorated the clinical signs of experimental autoimmune encephalitis induced by MOG peptide injection in a murine model. These results confirmed that tDCs with potent tolerogenic properties could be efficiently generated by the combined use of minocycline and dexamethasone, along with GM-CSF and IL-4. Our results would help in the development of ex vivo tDC-based immunotherapies. |
| Databáze: | OpenAIRE |
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