Human Chr18 transcriptome dataset combined from the Illumina HiSeq, ONT MinION, and qPCR data
Autor: | Y.Y. Kiseleva, Anastasia V. Tsvetkova, I.V. Buromski, I. V. Vakhrushev, Elena A. Ponomarenko, L. K. Kurbatov, Olga S. Timoshenko, Svetlana A. Khmeleva, Ekaterina V. Ilgisonis, Alexander I. Archakov, Sergey P. Radko, Timur Shkrigunov, Andrey Lisitsa, Sergey S. Markin, Olga I. Kiseleva, K.G. Ptitsyn, George S. Krasnov, V.V. Shapovalova |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
HepG2
Science (General) Computer applications to medicine. Medical informatics R858-859.7 Computational biology Biology Transcriptome 03 medical and health sciences Q1-390 0302 clinical medicine Liver tissue Gene expression level Human proteome project Transcriptome profiling 030304 developmental biology Data Article 0303 health sciences Oxford nanopore Technologies Multidisciplinary Illumina HiSeq Transcriptome Sequencing Minion Nanopore sequencing 030217 neurology & neurosurgery |
Zdroj: | Data in Brief, Vol 36, Iss, Pp 107130-(2021) Data in Brief |
ISSN: | 2352-3409 |
Popis: | The chromosome-centric dataset was created by applying several technologies of transcriptome profiling. The described dataset is available at NCBI repository (BioProject ID PRJNA635536). The dataset referred to the same type of tissue, cell lines, transcriptome sequencing technologies, and was accomplished in a period of 8 years (the first data were obtained in 2013 while the last ones - in 2020). The high-throughput sequencing technologies were employed along with the quantitative PCR (qPCR) approach, for data generation using the gene expression level assessment. qPCR was performed for a limited group of genes, encoded on human chromosome 18, for the Russian part of the Chromosome-Centric Human Proteome Project. The data of high-throughput sequencing are provided as Excel spreadsheets, where the data on FPKM and TMP values were evaluated for the whole transcriptome with both Illumina HiSeq and Oxford Nanopore Technologies MinION sequencing. |
Databáze: | OpenAIRE |
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