Flow cytometric findings in platelets of patients following allogeneic hematopoietic stem cell transplantation
| Autor: | Kolb Hj, Erhard Hiller, Christoph Salat, H Wegner, Ernst Holler, M Pihusch, R. Pihusch |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Blood Platelets Male Pathology medicine.medical_specialty medicine.medical_treatment Graft vs Host Disease Platelet Membrane Glycoproteins Hematopoietic stem cell transplantation Cell Degranulation Flow cytometry immune system diseases hemic and lymphatic diseases Immunopathology Humans Thrombophilia Transplantation Homologous Medicine Platelet Survivors Receptor Transplantation medicine.diagnostic_test business.industry Hematopoietic Stem Cell Transplantation Degranulation Hematology Middle Aged Flow Cytometry Platelet Activation Hematologic Diseases surgical procedures operative Case-Control Studies Immunology Female Stem cell business Complication |
| Zdroj: | Bone Marrow Transplantation. 30:381-387 |
| ISSN: | 1476-5365 0268-3369 |
| DOI: | 10.1038/sj.bmt.1703663 |
| Popis: | Following allogeneic hematopoietic stem cell transplantation (HSCT) patients may have an increased bleeding tendency in spite of a normal platelet count. Moreover, an association between chronic graft-versus-host disease (cGVHD) and a thrombophilic state has been observed. Platelet receptors and granules from 27 patients following HSCT (13 without cGVHD, 14 with cGVHD) were evaluated by flow cytometric analysis and compared to 62 healthy controls. Platelets from HSCT patients stained weakly with mepacrine indicating a reduced content of dense bodies, whereas no significant degranulation reaction of alpha granules and lysosomes was detectable. In addition, a lower surface expression of GP Ia/IIa was observed, indicating an acquired thrombocytopathy. The surface receptors are activated in HSCT patients, which could be seen by the lower surface expression of GP Ib internalized during the activation process and elevated levels of LIBS-1 and PAC-1 antibody binding. Patients with cGVHD had a seven-fold increased ratio of microparticles. This study demonstrates platelet receptor and granule defects in patients following HSCT. The key role of platelets in HSCT-associated hemostatic disorders is underscored by the high levels of circulating microparticles in cGvHD patients which might explain the thrombophilic state in these patients. |
| Databáze: | OpenAIRE |
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