Immunoassay as an Analytical Tool in Agricultural Biotechnology
| Autor: | Randal Giroux, James W Stave, Virginia Pantella, Murali Bandla, Guomin Shan, Thomas Currier, G David Grothaus, Markus Lipp, G. Ronald Jenkins |
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| Rok vydání: | 2006 |
| Předmět: |
Crops
Agricultural Computer science Supply chain Food Genetically Modified Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Antibodies Chemistry Techniques Analytical Analytical Chemistry medicine Environmental Chemistry Production (economics) Product (category theory) Antigens Plant Proteins Immunoassay Pharmacology medicine.diagnostic_test business.industry Diagnostic test Agriculture Agricultural biotechnology Plants Genetically Modified Food Analysis Biotechnology New product development Biochemical engineering Genetic Engineering business Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 89:913-928 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/89.4.913 |
| Popis: | Immunoassays for biotechnology engineered proteins are used by AgBiotech companies at numerous points in product development and by feed and food suppliers for compliance and contractual purposes. Although AgBiotech companies use the technology during product development and seed production, other stakeholders from the food and feed supply chains, such as commodity, food, and feed companies, as well as third-party diagnostic testing companies, also rely on immunoassays for a number of purposes. The primary use of immunoassays is to verify the presence or absence of genetically modified (GM) material in a product or to quantify the amount of GM material present in a product. This article describes the fundamental elements of GM analysis using immunoassays and especially its application to the testing of grains. The 2 most commonly used formats are lateral flow devices (LFD) and plate-based enzyme-linked immunosorbent assays (ELISA). The main applications of both formats are discussed in general, and the benefits and drawbacks are discussed in detail. The document highlights the many areas to which attention must be paid in order to produce reliable test results. These include sample preparation, method validation, choice of appropriate reference materials, and biological and instrumental sources of error. The article also discusses issues related to the analysis of different matrixes and the effects they may have on the accuracy of the immunoassays. |
| Databáze: | OpenAIRE |
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