Identification and culture of olfactory neural progenitors from GFP mice
| Autor: | Fred J. Roisen, Othman Mm, Kathleen M. Klueber |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Green Fluorescent Proteins Population Cell Culture Techniques Mice Transgenic Cell Separation Biology Green fluorescent protein Mice Olfactory mucosa Olfactory Mucosa Neurosphere medicine Animals Tissue Distribution education Cells Cultured Neurons education.field_of_study Stem Cells Cell Differentiation General Medicine Zinc Sulfate Cell biology Transplantation Luminescent Proteins Medical Laboratory Technology medicine.anatomical_structure Microscopy Fluorescence Stem cell Olfactory epithelium Adult stem cell |
| Zdroj: | Biotechnic & Histochemistry. 78:57-70 |
| ISSN: | 1473-7760 1052-0295 |
| DOI: | 10.1080/10520290310001593801 |
| Popis: | The olfactory epithelium (OE) is one of the best sources for obtaining adult stem cells from the nervous system, because it contains neural progenitors that regenerate continuously throughout life. The OE is accessible through the nasal cavity, which facilitates stem cell harvest for examination and transplantation. The mitotic activity of OE progenitors can be stimulated by intranasal irrigation with zinc sulfate (ZnSO4). In the study reported here, we focused on OE from a transgenic mouse line transfected with green fluorescent protein (GFP). Histological examination demonstrated the site of highest yield of OE in the transgenic and wild type littermates. Cultures were established from that site four days in vitro following ZnSO4 exposure. The GFP-derived primary cultures contained a heterogeneous population of fluorescent cells. After 10-12 days, a population of round, mitotically active cells emerged that formed fluorescent neurospheres. The neurosphere forming cells (NSFCs) were collected and subcultured up to four times. The NSFCs were primarily neuronal with only a few cells of glial lineage. Furthermore, the NSFCs were nestin positive and keratin negative, suggesting that they were neural progenitors. The endogenous GFP fluorescence of these cells provides a readily identifiable label that will facilitate their identification following transplantation into nontransfected hosts. They should provide a useful model for evaluating the potential therapeutic utility of OE progenitors in neurodegenerative diseases and neurotrauma repair. |
| Databáze: | OpenAIRE |
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