Production of single-chain fragment variable (scFv) antibodies specific to plasma membrane epitopes on bull Y-bearing sperm
| Autor: | Wiwat Pattanawong, Surat Hongsibsong, Korawan Sringarm, Supamit Mekchay, Wannaluk Thaworn, Marninphan Thongkham |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
endocrine system animal diseases Bioengineering Single chain Biology Epitope Epitopes fluids and secretions Animals ScFv Antibodies Cloning Molecular reproductive and urinary physiology Hybridomas urogenital system Fragment (computer graphics) Cell Membrane Antibodies Monoclonal Bull semen Spermatozoa Sperm Molecular biology Membrane Cattle Animal Science and Zoology Single-Chain Antibodies Biotechnology |
| Zdroj: | Animal Biotechnology. 33:508-518 |
| ISSN: | 1532-2378 1049-5398 |
| DOI: | 10.1080/10495398.2020.1811294 |
| Popis: | Distinguishing between bull Y- and X-bearing sperm populations is advantageous for techniques using sexed bull semen. The aim of this study was to produce a single-chain fragment variable (scFv) antibody against plasma membrane epitopes on bull Y-bearing sperm. Variable heavy (VH)- and variable light (VL)-region genes generated from a hybridoma cell secreting a specific Y-bearing sperm monoclonal antibody (mAb-1F9) were cloned and expressed. The expected sizes of the DNA bands were ∼350 bp for the VH gene and ∼318 bp for the VL gene. The VH and VL genes were generated and used to construct an scFv gene (∼650 bp), which was expressed in |
| Databáze: | OpenAIRE |
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