| Autor: |
Sandeep Pawar, Amit Mirgal, Sonali S. Bharate, Aboli Girme, Saurabh Patel, Ganesh Saste, D. Srinivasa Reddy, Anshuly Tiwari, Sautik Ghoshal, Lal Hingorani, Chetana Ghule, Ram A. Vishwakarma, Sandip B. Bharate |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
ACS Omega, Vol 6, Iss 36, Pp 23460-23474 (2021) |
| ISSN: |
2470-1343 |
| Popis: |
Food ingredients hold a higher nutritional value as a botanical supplement playing a vital role in modifying and maintaining the physiological conditions that improve human health benefits. The Kashmir saffron (Crocus sativus L; KCS) obtained from dried stigmas is known for its aroma precursors and apocarotenoid derivatives, imparting a wide range of medicinal values and therapeutic benefits. In the present study, a simultaneous determination of apocarotenoids and flavonoids in stigma-based botanical supplements was carried out using analytical investigations. The high-performance thin-layer chromatography-based qualitative analysis of the raw material (stigmas, stamens, and tepals) and stigma extract has been carried out to identify apocarotenoids and flavonoids. The rapid HPLC-PDA method for the simultaneous quantification of KCS apocarotenoids was robust, precise ( 0.99), and accurate (80-110%) as per the single-laboratory validation data. Furthermore, the combined-expanded uncertainty (95%; K = 2) was calculated and found as 0.0035-0.007% ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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