The Effect of Sulforaphane on Glyoxalase I Expression and Activity in Peripheral Blood Mononuclear Cells
| Autor: | Michela Alfarano, Teresa Oliviero, Vincenzo Fogliano, Casper G. Schalkwijk, Donato Pastore |
|---|---|
| Přispěvatelé: | Interne Geneeskunde, RS: CARIM - R3.01 - Vascular complications of diabetes and the metabolic syndrome |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine CHEMOPREVENTION DEFENSE sulforaphane lcsh:TX341-641 ISOTHIOCYANATES Brassica Peripheral blood mononuclear cell Article glyoxalase 1 MECHANISMS Eating 03 medical and health sciences chemistry.chemical_compound Lactoylglutathione lyase Glycation Gene expression Humans glutathione Glutathione Transferase VLAG Nutrition and Dietetics biology INDUCTION Methylglyoxal Lactoylglutathione Lyase glutathione-S-transferase Glutathione Middle Aged peripheral blood mononuclear cells Molecular biology Food Quality and Design 030104 developmental biology Glutathione S-transferase chemistry Sulfoxides METHYLGLYOXAL Leukocytes Mononuclear biology.protein Female GLYCATION END-PRODUCTS lcsh:Nutrition. Foods and food supply SYSTEM Food Science Sulforaphane |
| Zdroj: | Nutrients, 10(11):1773. Multidisciplinary Digital Publishing Institute (MDPI) Nutrients 10 (2018) 11 Nutrients Volume 10 Issue 11 Nutrients, Vol 10, Iss 11, p 1773 (2018) Nutrients, 10(11) |
| ISSN: | 2072-6643 |
| Popis: | Studies demonstrate that the potential health-beneficial effect of sulforaphane (SR), a compound formed in broccoli, is the result of a number of mechanisms including upregulation of phase two detoxification enzymes. Recent studies suggest that SR increases expression/activity of glyoxalase 1 (Glo1), an enzyme involved in the degradation of methylglyoxal, is major precursor of advanced glycation end products. Those compounds are associated with diabetes complications and other age-related diseases. In this study, the effect of SR on the expression/activity of Glo1 in peripheral blood mononuclear cells (PBMCs) from 8 healthy volunteers was investigated. PBMCs were isolated and incubated with SR (2.5 &mu M-concentration achievable by consuming a broccoli portion) for 24 h and 48 h. Glo1 activity/expression, reduced glutathione (GSH), and glutathione-S-transferase gene expression were measured. Glo1 activity was not affected while after 48 h a slight but significant increase of its gene expression (1.03-fold) was observed. GSTP1 expression slightly increased after 24 h incubation (1.08-fold) while the expressions of isoform GSTT2 and GSTM2 were below the limit of detection. GSH sharply decreased, suggesting the formation of GSH-SR adducts that may have an impact SR availability. Those results suggest that a regular exposure to SR by broccoli consumption or SR supplements may enhance Glo1. |
| Databáze: | OpenAIRE |
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