Fractionation of human liver mitochondria: Enzymic and morphological characterization of the inner and outer membranes as compared to RAT liver mitochondria

Autor:	R. Tilinca, Victor I. Pop, S. Dancea, John M. Wrigglesworth, Gheorghe Benga, D. Porutiu, A. Hodarnau
Rok vydání:	1979
Předmět:	Submitochondrial Particles Mitochondria Liver Cell Biology Fractionation Mitochondrion Matrix (biology) Biology Cell Fractionation Rats Microscopy Electron chemistry.chemical_compound Digitonin Membrane chemistry Biochemistry Animals Humans Inner membrane Centrifugation Bacterial outer membrane
Zdroj:	Journal of Cell Science. 35:417-429
ISSN:	1477-9137 0021-9533
DOI:	10.1242/jcs.35.1.417
Popis:	The fractionation of human liver mitochondria into inner membrane, outer membrane and matrix material is reported. Compared with rat, human liver mitochondria are more fragile. Fractionation can be achieved in only 2 steps, a digitonin treatment for removal of the outer membrane and centrifugation of the inner membrane plus matrix particles through a linear sucrose gradient resulting in purified inner membranes and matrix.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f553fb5b4297ef6eba1e930ed74e95cf https://doi.org/10.1242/jcs.35.1.417 Zobrazit plný text záznamu