Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay
| Autor: | Shao-Hui Liang, Xiao-Guang Chen, Lin Ai, Mu-Xin Chen, Rui-Qing Lin, Xi-Mei Zhan, Min-Jun Xu, Chunyan Liu, Hui-Qun Song, Zi-Guo Yuan, Xing-Quan Zhu, Renli Zhang |
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| Rok vydání: | 2011 |
| Předmět: |
Trichinella spiralis
Snails Loop-mediated isothermal amplification DNA Ribosomal Polymerase Chain Reaction Sensitivity and Specificity law.invention Microbiology law parasitic diseases medicine Animals Molecular Biology Polymerase chain reaction DNA Primers Strongylida Infections biology Angiostrongylus cantonensis Cell Biology Nucleic acid amplification technique DNA Helminth biology.organism_classification medicine.disease Molecular biology Larva Angiostrongyliasis Lungworm Nucleic Acid Amplification Techniques Toxocara canis |
| Zdroj: | Molecular and cellular probes. 25(4) |
| ISSN: | 1096-1194 |
| Popis: | Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten by humans in some countries. In the present study, we developed a loop-mediated isothermal amplification (LAMP) method for the specific detection of A. cantonensis in Ac. fulica. Primers for LAMP were designed based on the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA (rDNA) of A. cantonensis. Specificity tests showed that only the products of A. cantonensis were detected when DNA samples of A. cantonensis and the heterologous control samples Anisakis simplex s.s, Trichuris trichiura, Toxocara canis, Trichinella spiralis and Ascaris lumbricoides were amplified by LAMP. Sensitivity evaluation indicated that the LAMP assay is 10 times more sensitive than the conventional polymerase chain reaction (PCR) assay. The established LAMP assay is rapid, inexpensive and easy to be performed. It can be used in clinical applications for rapid and sensitive detection of A. cantonensis in snails, which has implications for the effective control of angiostrongyliasis. |
| Databáze: | OpenAIRE |
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