Comparison of fluorescent stains: Relative photostability and differential staining of proteins in two-dimensional gels
| Autor: | Gary B. Smejkal, Myra H. Robinson, Alexander Lazarev |
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| Rok vydání: | 2004 |
| Předmět: |
Two-dimensional gel electrophoresis
Chromatography Staining and Labeling Differential staining Coomassie Brilliant Blue Clinical Biochemistry Epicocconone Proteins Transillumination Biochemistry Molecular biology Stain Fluorescence Analytical Chemistry Silver stain chemistry.chemical_compound chemistry Escherichia coli Image Processing Computer-Assisted Electrophoresis Gel Two-Dimensional Fluorescent Dyes |
| Zdroj: | ELECTROPHORESIS. 25:2511-2519 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.200406005 |
| Popis: | The fluorescence of proteins stained with Deep Purple and SYPRO Ruby was measured over a time course of UV transillumination to determine the relative photostability of each stain. Mean spot fluorescence (n = 200 matched spots) in gels stained with Deep Purple decreased 27% following 2 min of UV transillumination, compared to SYPRO Ruby, which decreased 17%. After 19 min, an 83% decrease in Deep Purple fluorescence was observed, compared to 44% for SYPRO Ruby. By interpolation, the half-life of Deep Purple fluorescence was estimated to be approximately 6 min. The half-life of SYPRO Ruby fluorescence was not reached during the 19 min time course. Further, differential staining of proteins was observed in gels stained with Deep Purple and SYPRO Ruby as compared to colloidal Coomassie Brilliant Blue and silver staining. |
| Databáze: | OpenAIRE |
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