Autograft microskin combined with adipose-derived stem cell enhances wound healing in a full-thickness skin defect mouse model

Autor:	Ying Hu, Ronghan He, Tangzhao Liang, Yuansen Luo, Lei Zhu, Xiaoyou Yi, Kun Wang, Shihai Jiang, Li Bai, Chunmei Wang
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Male Proteomics 0301 basic medicine CD31 Angiogenesis medicine.medical_treatment Medicine (miscellaneous) Wound healing Adipose-derived stem cell Transplantation Autologous Biochemistry Genetics and Molecular Biology (miscellaneous) lcsh:Biochemistry Mice 03 medical and health sciences Paracrine signalling 0302 clinical medicine Adipocytes Animals Transplantation Homologous Medicine lcsh:QD415-436 Autografts Skin Secretome Mice Inbred BALB C lcsh:R5-920 Full-thickness skin defect integumentary system business.industry Stem Cells Research Skin Transplantation Cell Biology Urokinase receptor Transplantation Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Molecular Medicine Skin grafting Stem cell business lcsh:Medicine (General) Microskin
Zdroj:	Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-15 (2019) Stem Cell Research & Therapy
ISSN:	1757-6512
DOI:	10.1186/s13287-019-1389-4
Popis:	Objective Autograft microskin transplantation has been widely used as a skin graft therapy in full-thickness skin defect. However, skin grafting failure can lead to a pathological delay wound healing due to a poor vascularization bed. Considering the active role of adipose-derived stem cell (ADSC) in promoting angiogenesis, we intend to investigate the efficacy of autograft microskin combined with ADSC transplantation for facilitating wound healing in a full-thickness skin defect mouse model. Material and methods An in vivo full-thickness skin defect mouse model was used to evaluate the contribution of transplantation microskin and ADSC in wound healing. The angiogenesis was detected by immunohistochemistry staining. In vitro paracrine signaling pathway was evaluated by protein array and Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway, and protein-protein interaction network analysis. Results Co-transplantation of microskin and ADSC potentiated the wound healing with better epithelization, smaller scar thickness, and higher angiogenesis (CD31) in the subcutaneous layer. We found both EGF and VEGF cytokines were secreted by microskin in vitro. Additionally, secretome proteomic analysis in a co-culture system of microskin and ADSC revealed that ADSC could secrete a wide range of important molecules to form a reacting network with microskin, including VEGF, IL-6, EGF, uPAR, MCP-3, G-CSF, and Tie-2, which most likely supported the angiogenesis effect as observed. Conclusion Overall, we concluded that the use of ADSC partially modulates microskin function and enhances wound healing by promoting angiogenesis in a full-thickness skin defect mouse model. Electronic supplementary material The online version of this article (10.1186/s13287-019-1389-4) contains supplementary material, which is available to authorized users.
Databáze:	OpenAIRE
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