Metastasis of breast cancer cells to the bone, lung, and lymph nodes promotes resistance to ionizing radiation
| Autor: | Seiichi Takenoshita, Manabu Iwadate, Nobuyuki Hamada, Satoshi Waguri, Kazunoshin Tachibana, Takamitsu Hara |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CA15-3 Pathology medicine.medical_specialty Lung Neoplasms medicine.medical_treatment Mice Nude Bone Neoplasms Breast Neoplasms Radiation Tolerance Metastasis Mice 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Line Tumor Radioresistance Animals Humans Medicine Bioluminescence imaging Radiology Nuclear Medicine and imaging Clonogenic assay Mice Inbred BALB C business.industry Cancer Dose-Response Relationship Radiation Radiotherapy Dosage medicine.disease Radiation therapy 030104 developmental biology Oncology Lymphatic Metastasis 030220 oncology & carcinogenesis Female business |
| Zdroj: | Strahlentherapie und Onkologie. 193:848-855 |
| ISSN: | 1439-099X 0179-7158 |
| Popis: | Metastasis represents the leading cause of breast cancer deaths, necessitating strategies for its treatment. Although radiotherapy is employed for both primary and metastatic breast cancers, the difference in their ionizing radiation response remains incompletely understood. This study is the first to compare the radioresponse of a breast cancer cell line with its metastatic variants and report that such metastatic variants are more radioresistant. A luciferase expressing cell line was established from human basal-like breast adenocarcinoma MDA-MB-231 and underwent in vivo selections, whereby a cycle of inoculations into the left cardiac ventricle or the mammary fat pad of athymic nude mice, isolation of metastases to the bone, lung and lymph nodes visualized with bioluminescence imaging, and expansion of obtained cells was repeated twice or three times. The established metastatic cell lines were assessed for cell proliferation, wound healing, invasion, clonogenic survival, and apoptosis. The established metastatic cell lines possessed an increased proliferative potential in vivo and were more chemotactic, invasive, and resistant to X‑ray-induced clonogenic inactivation and apoptosis in vitro. Breast cancer metastasis to the bone, lung, and lymph nodes promotes radioresistance. |
| Databáze: | OpenAIRE |
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