Circadian disruption by shifting the light-dark cycle negatively affects bone health in mice

Autor: Björn Busse, Kathrin Mletzko, Nathalie Bravenboer, Maaike Schilperoort, Leo van Ruijven, Patrick C.N. Rensen, Sander Kooijman, Joann Lim, Jan Kroon, Elizabeth M. Winter
Přispěvatelé: Oral Cell Biology
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
bone turnover
Light
Cathepsin K
Osteoclast proliferation
CLOCK Proteins
Biochemistry
Bone remodeling
Mice
0302 clinical medicine
Bone Density
Osteogenesis
Behavior
Animal
ARNTL Transcription Factors
Period Circadian Proteins
Lipids
CLOCK
PER2
medicine.anatomical_structure
RANKL
Osteocyte
Female
Proto-Oncogene Proteins c-fos
Biotechnology
PER1
circadian rhythm
medicine.medical_specialty
bone structure
Photoperiod
Biology
Bone and Bones
03 medical and health sciences
Internal medicine
Circadian Clocks
Genetics
medicine
clock genes
Animals
Circadian rhythm
Molecular Biology
RANK Ligand
Osteoprotegerin
X-Ray Microtomography
Cryptochromes
030104 developmental biology
Endocrinology
Gene Expression Regulation
bone mineralization
Nuclear Receptor Subfamily 1
Group D
Member 1
biology.protein
030217 neurology & neurosurgery
Zdroj: FASEB Journal, 34(1), 1052-1064. FEDERATION AMER SOC EXP BIOL
The FASEB Journal, 34(1), 1052-1064. FASEB
Schilperoort, M, Bravenboer, N, Lim, J, Mletzko, K, Busse, B, van Ruijven, L, Kroon, J, Rensen, P C N, Kooijman, S & Winter, E M 2020, ' Circadian disruption by shifting the light-dark cycle negatively affects bone health in mice ', The FASEB Journal, vol. 34, no. 1, pp. 1052-1064 . https://doi.org/10.1096/fj.201901929R
ISSN: 0892-6638
DOI: 10.1096/fj.201901929R
Popis: The past decade, it has become evident that circadian rhythms within metabolically active tissues are very important for physical health. However, although shift work has also been associated with an increased risk of fractures, circadian rhythmicity has not yet been extensively studied in bone. Here, we investigated which genes are rhythmically expressed in bone, and whether circadian disruption by shifts in light-dark cycle affects bone turnover and structure in mice. Our results demonstrate diurnal expression patterns of clock genes (Rev-erbα, Bmal1, Per1, Per2, Cry1, Clock), as well as genes involved in osteoclastogenesis, osteoclast proliferation and function (Rankl, Opg, Ctsk), and osteocyte function (c-Fos) in bone. Weekly alternating light-dark cycles disrupted rhythmic clock gene expression in bone and caused a reduction in plasma levels of procollagen type 1 amino-terminal propeptide (P1NP) and tartrate-resistant acidic phosphatase (TRAP), suggestive of a reduced bone turnover. These effects coincided with an altered trabecular bone structure and increased cortical mineralization after 15 weeks of light-dark cycles, which may negatively affect bone strength in the long term. Collectively, these results show that a physiological circadian rhythm is important to maintain bone health, which stresses the importance of further investigating the association between shift work and skeletal disorders.
Databáze: OpenAIRE
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