Glutamate-like Immunoreactivity in Retinal Terminals in the Nucleus of the Optic Tract in Rabbits
| Autor: | B. Nunes Cardozo, R.M. Buijs, J. J. L. van der Want |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN) |
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Male
Retinal Ganglion Cells Superior Colliculi Optic tract Wheat Germ Agglutinins Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate Glutamic Acid Dendrite Biology Axonal Transport Nerve Fibers Myelinated Immunoenzyme Techniques Glutamates Neuropil medicine Animals Visual Pathways Pretectal area Microscopy Immunoelectron Horseradish Peroxidase gamma-Aminobutyric Acid Retina General Neuroscience Glutamate receptor Anatomy Axons Anterograde tracing medicine.anatomical_structure nervous system Retinal ganglion cell Synapses Biophysics sense organs Rabbits |
| Zdroj: | Journal of Comparative Neurology, 309, 261-270. Wiley-Liss Inc. |
| ISSN: | 0021-9967 |
| Popis: | The ultrastructural organization of retinal terminals within the nucleus of the optic tract of rabbits was investigated with a combination of anterograde tracing and immunocytochemistry. The anterogradely transported WGA-HRP injected in the vitreous of the eye was visualized with the sensitive gold-substituted silver peroxidase (GSSP) method. Glutamate and GABA immunoreactivity were identified with postembedding colloidal gold particles. Retinal ganglion cell terminals (R-terminals) in the nucleus of the optic tract formed asymmetric synapses and contained spherical vesicles and electron lucent mitochondria. R-terminals were observed in large clusters in the neuropil and in synaptic contact with large initial dendrites and somata. Within the clusters of neuropil the R-terminals formed two types of glomeruluslike arrangements: (1) an R-terminal centrally located and surrounded by small dendritic and axonal profiles and (2) several R-terminals surrounding a single dendrite or a group of dendritic profiles, presumably of interneuronal origin. All R-terminals identified with WGA-HRP as well as those exhibiting similar ultrastructural characteristics showed high levels of glutamate immunoreactivity, but no GABA immunoreactivity. The presence of glutamate and the absence of GABA in R-terminals suggest that glutamate is involved in neurotransmission in the pathway from retina to the nucleus of the optic tract of rabbits. |
| Databáze: | OpenAIRE |
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