Long‐Term Imaging of Wound Angiogenesis with Large Scale Optoacoustic Microscopy
| Autor: | Daniel Razansky, Sabine Werner, Maya Ben-Yehuda Greenwald, Mateusz S. Wietecha, Johannes Rebling |
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| Přispěvatelé: | University of Zurich, Werner, Sabine, Razansky, Daniel |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Angiogenesis
medicine.medical_treatment General Chemical Engineering 10050 Institute of Pharmacology and Toxicology General Physics and Astronomy Medicine (miscellaneous) Genetics and Molecular Biology (miscellaneous) Regenerative medicine Biochemistry 170 Ethics 030207 dermatology & venereal diseases Mice 0302 clinical medicine Tissue engineering General Materials Science 0303 health sciences Microscopy Full Paper General Engineering 2701 Medicine (miscellaneous) Full Papers 3100 General Physics and Astronomy 3. Good health Models Animal Female Preclinical imaging Intravital microscopy skin Intravital intravital microscopy microcirculation photoacoustic sola cutis se reficientis vascularization Science Neovascularization Physiologic 610 Medicine & health Revascularization Biochemistry Genetics and Molecular Biology (miscellaneous) 1301 Biochemistry Genetics and Molecular Biology (miscellaneous) Microcirculation Photoacoustic Techniques 03 medical and health sciences medicine Animals 10237 Institute of Biomedical Engineering 1500 General Chemical Engineering 030304 developmental biology Wound Healing business.industry 2500 General Materials Science intravital 2200 General Engineering business Wound healing Biomedical engineering |
| Zdroj: | Advanced Science, Vol 8, Iss 13, Pp n/a-n/a (2021) Advanced Science Advanced Science, 8 (13) |
| Popis: | Wound healing is a well‐coordinated process, necessitating efficient formation of new blood vessels. Vascularization defects are therefore a major risk factor for chronic, non‐healing wounds. The dynamics of mammalian tissue revascularization, vessel maturation, and remodeling remain poorly understood due to lack of suitable in vivo imaging tools. A label‐free large‐scale optoacoustic microscopy (LSOM) approach is developed for rapid, non‐invasive, volumetric imaging of tissue regeneration over large areas spanning up to 50 mm with a depth penetration of 1.5 mm. Vascular networks in dorsal mouse skin and full‐thickness excisional wounds are imaged with capillary resolution during the course of healing, revealing previously undocumented views of the angiogenesis process in an unperturbed wound environment. Development of an automatic analysis framework enables the identification of key features of wound angiogenesis, including vessel length, diameter, tortuosity, and angular alignment. The approach offers a versatile tool for preclinical research in tissue engineering and regenerative medicine, empowering label‐free, longitudinal, high‐throughput, and quantitative studies of the microcirculation in processes associated with normal and impaired vascular remodeling, and analysis of vascular responses to pharmacological interventions in vivo. Large‐scale optoacoustic microscopy is presented for long‐term imaging of vascular dynamics during wound healing in an unperturbed in vivo environment. The new method offers a versatile tool for preclinical research in tissue engineering and regenerative medicine, empowering label‐free, noninvasive, longitudinal, high‐throughput, and quantitative studies of microcirculation in processes associated with vascular remodeling, and analysis of vascular responses to pharmacological interventions. |
| Databáze: | OpenAIRE |
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