Boron enhances early embryonic gene expressions and improves fetal development of rats
| Autor: | Gamze Dal, Sinan Ince, Metin Erdogan, Yuksel Agca, Hasan Hüseyin Demirel, Cevdet Uguz |
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| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
0301 basic medicine Homeobox protein NANOG medicine.medical_specialty chemistry.chemical_element Real-Time Polymerase Chain Reaction Biochemistry Inorganic Chemistry Andrology Rats Sprague-Dawley 03 medical and health sciences In vivo medicine Animals RNA Messenger Boron Homeodomain Proteins Fetus Reverse Transcriptase Polymerase Chain Reaction Embryo Metabolism Nanog Homeobox Protein Embryonic stem cell Rats 030104 developmental biology chemistry Molecular Medicine Histopathology Female Octamer Transcription Factor-3 |
| Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 50 |
| ISSN: | 1878-3252 |
| Popis: | Boron is present as several different components in nature. Besides its industrial use, it is an essential element and is playing a very important role in the metabolism. In this study, it was aimed to determine the in vivo effects of boron on mRNA expression of HEX, NANOG, and OCT-3/4 genes in embryo and histological changes during fetal development. Therefore, totally 60 female rats were allocated into 5 equal groups. Experimental groups are as the followings; positive control (fed with standart rat diet), negative control (fed with boron free diet), low boron group (fed with boron free diet and given 0.04 μg boron/ml via gastric gavage), marginal boron group (fed with boron free diet and given 0.3 μg boron/ml via gastric gavage) and normal boron group (fed with boron free diet and given 2 μg boron/ml via gastric gavage). Experimental period was performed for 14 days. Embryos were collected after 4 days of mating and the expression and protein levels of early embryonic genes namely HEX, NANOG, and OCT-3/4 were determined by using Real-Time PCR. Also, 10–20 day embryo and fetus development were histologically determined. According to the results, mRNA expression and protein levels of early embryonic genes were increased in boron groups while decreased in boron deficient group. Histopathologically, tissue and organ developments were definitely observed in the boron groups. In conclusion, mRNA expression levels of early embryonic genes decreased in boron deficient group and boron has an important role for fetal development. |
| Databáze: | OpenAIRE |
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