Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes
| Autor: | Birgit Dietzel, Roland Ludwig, Burkhard Schulz, Frieder W. Scheller, Daniel Kracher, Johannes Tanne, Frank F. Bier, Fred Lisdat |
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| Přispěvatelé: | Fraunhofer Institute for Cell Therapy and Immunology (IZI-BB), Branch Bioanalytics and Bioprocesses Potsdam-Golm, Food Biotechnology Laboratory, Department of Food Sciences and Technology, BOKU-University of Natural Resources and Life Sciences, Institute for Thin Film and Microsensoric Technology |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cellobiose dehydrogenase
Chemistry lcsh:Biotechnology Clinical Biochemistry Nanotechnology General Medicine Carbon nanotube multiwalled carbon nanotubes Ascorbic acid Amperometry Article polyaniline law.invention nanohybrids chemistry.chemical_compound law Covalent bond ddc:570 lcsh:TP248.13-248.65 Polyaniline Surface charge Fourier transform infrared spectroscopy cellobiose dehydrogenase Nuclear chemistry |
| Zdroj: | Biosensors, Vol 4, Iss 4, Pp 370-386 (2014) Biosensors Biosensors, 4, 4, S. 370-386 Volume 4 Issue 4 Pages 370-386 |
| ISSN: | 2079-6374 |
| Popis: | Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently linked to the surface of MWCNTs while modification with p-phenylenediamine (PDA) converted the COOH-groups to positively charged amino groups. Fourier transform infrared spectroscopy (FTIR) measurements verified the p-phenylenediamine (PDA) modification of the polymer-CNT nanohybrids. The positively charged nanohybrid MWCNT-P(AMB-A)-PDA promoted direct electron transfer (DET) of CDH to the electrode and bioelectrocatalysis of lactose was observed. Amperometric measurements gave an electrochemical response with KMapp = 8.89 mM and a current density of 410 nA/cm(2) (15 mM lactose). The catalytic response was tested at pH 3.5 and 4.5. Interference by ascorbic acid was not observed. The study proves that DET between the MWCNT-P(AMB-A)-PDA nanohybrids and CDH is efficient and allows the sensorial detection of lactose. |
| Databáze: | OpenAIRE |
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