Salivary microRNAs identified by small RNA sequencing as potential predictors of response to intensity-modulated radiotherapy in head and neck cancer patients

Autor:	Natalia Anna Gablo, Bretislav Gal, Jiri Sana, Dominik Gurin, Markéta Hermanová, Tomas Kazda, Zuzana Horáková, Pavel Šlampa, Karolina Trachtova, Marek Slávik, Ondrej Slaby, Pavel Smilek, Parwez Ahmad
Rok vydání:	2020
Předmět:	Male 0301 basic medicine Oncology Cancer Research Saliva medicine.medical_specialty medicine.medical_treatment Cell 03 medical and health sciences 0302 clinical medicine Internal medicine microRNA Humans Medicine Prospective cohort study Aged Proportional Hazards Models Sequence Analysis RNA business.industry Proportional hazards model Head and neck cancer General Medicine Middle Aged medicine.disease Molecular diagnostics Progression-Free Survival Radiation therapy MicroRNAs stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Head and Neck Neoplasms 030220 oncology & carcinogenesis Molecular Medicine Female Radiotherapy Intensity-Modulated business
Zdroj:	Cellular Oncology. 43:505-511
ISSN:	2211-3436 2211-3428
DOI:	10.1007/s13402-020-00507-7
Popis:	Progress in radiation therapy of head and neck squamous cell carcinomas (HNSCCs) is logically linked to the development of molecular predictors that would help to enhance individually tailored treatment. MicroRNA (miRNA) expression profiles in tumors have repeatedly been tested to optimize the molecular diagnostics of HNSCC. In addition to tumor tissues, miRNAs are stably present in body fluids, including saliva, and can thus be collected non-invasively. The aim of our current study was to evaluate whether salivary miRNAs have potential as response predictors in HNSCC patients treated with intensity modulated radiation therapy (IMRT). In total 48 HNSCC patients treated by definitive IMRT were enrolled in our prospective study. To identify predictive salivary miRNAs, we used small RNA sequencing in 14 saliva samples of HNSCC patients and qRT-PCR validation of selected miRNA candidates in an independent set of 34 patients. We found that salivary miR-15a-5p and miR-15b-5p exhibited differential levels between patients with and without complete remission (p = 0.025 and p = 0.028, respectively). Subsequent Kaplan-Meier analysis confirmed that patients with higher levels of miR-15a-5p reached a significantly longer locoregional progression-free survival (LPFS) than those with low levels (p = 0.024). Finally, multivariate Cox regression analysis revealed that miR-15a-5p may serve as an independent predictive biomarker of LPFS in HNSCC patients treated with IMRT (HR 0.104; 95% CI 0.004–0.911; p = 0.04). We conclude that salivary miR-15a-5p may represent a potential biomarker for individualized treatment decision-making in HNSCC patients.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f53ae2e5187e99ec4d6a1dbb769537d3 https://doi.org/10.1007/s13402-020-00507-7 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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