Results of a Saxitoxin Proficiency Test Including Characterization of Reference Material and Stability Studies
| Autor: | Marc-André Avondet, Anke Kremp, Paula Vanninen, Martin Söderström, Martin Schär, Marja-Leena Rapinoja, Sanna Suikkanen, Werner Arnold, Stephen Burrell, Harri Kankaanpää, Kirsi Harju, Werner Luginbühl |
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| Přispěvatelé: | Department of Chemistry, VERIFIN |
| Rok vydání: | 2015 |
| Předmět: |
proficiency test
Laboratory Proficiency Testing toksiinit Health Toxicology and Mutagenesis 116 Chemical sciences lcsh:Medicine saxitoxin Proficiency test dinoflagellate Toxicology 01 natural sciences Mice chemistry.chemical_compound Tandem Mass Spectrometry poisoning toxins refinement validation panssarilevät 0303 health sciences extension saksitoksiinit hermomyrkyt Reference Standards improve Dinoflagellata Shellfish poisoning analyysi Dinoflagellida mussel testit Biology paralytic shellfish toxins Article spectrometry menetelmät 03 medical and health sciences medicine Animals Shellfish Poisoning Paralytic shellfish poisoning 030304 developmental biology Mytilus Saxitoxin Chromatography lcsh:R ta1183 010401 analytical chemistry official method(sm) 2005.06 Alexandrium ostenfeldii simpukat medicine.disease quantification 0104 chemical sciences paralytic shellfish poisoning toxins chemistry Reference sample Marine Toxins sinisimpukka Marine toxin Chromatography Liquid |
| Zdroj: | Toxins Toxins, Vol 7, Iss 12, Pp 4852-4867 (2015) Volume 7 Issue 12 Pages 4852-4867 |
| ISSN: | 2072-6651 |
| Popis: | A saxitoxin (STX) proficiency test (PT) was organized as part of the Establishment of Quality Assurance for the Detection of Biological Toxins of Potential Bioterrorism Risk (EQuATox) project. The aim of this PT was to provide an evaluation of existing methods and the European laboratories' capabilities for the analysis of STX and some of its analogues in real samples. Homogenized mussel material and algal cell materials containing paralytic shellfish poisoning (PSP) toxins were produced as reference sample matrices. The reference material was characterized using various analytical methods. Acidified algal extract samples at two concentration levels were prepared from a bulk culture of PSP toxins producing dinoflagellate Alexandrium ostenfeldii. The homogeneity and stability of the prepared PT samples were studied and found to be fit-for-purpose. Thereafter, eight STX PT samples were sent to ten participating laboratories from eight countries. The PT offered the participating laboratories the possibility to assess their performance regarding the qualitative and quantitative detection of PSP toxins. Various techniques such as official Association of Official Analytical Chemists (AOAC) methods, immunoassays, and liquid chromatography-mass spectrometry were used for sample analyses. |
| Databáze: | OpenAIRE |
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