Flow cytometric diagnosis of partial mole
| Autor: | F Mangili, G. Marelli, I. Sassi, R. Marabini, A. Cantaboni, M. Patetta |
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| Rok vydání: | 1992 |
| Předmět: |
Pathology
medicine.medical_specialty Biology Trophoblastic Neoplasms Partial mole Pathology and Forensic Medicine Flow cytometry Diagnosis Differential Pregnancy medicine Humans Choriocarcinoma reproductive and urinary physiology Partial Hydatidiform Mole Ploidies medicine.diagnostic_test Karyotype Cell Biology DNA Neoplasm Hydatidiform Mole medicine.disease Flow Cytometry Hydatidiform moles Karyotyping embryonic structures Uterine Neoplasms Persistent trophoblastic disease Female Ploidy |
| Zdroj: | Pathology, research and practice. 188(4-5) |
| ISSN: | 0344-0338 |
| Popis: | To differentiate histologically partial hydatidiform moles (PM) and complete hydatidiform moles (CM) may be difficult. Cytogenetic studies have shown that PMs often had a triploid karyotype while CMs were always diploid. We assessed the DNA content of 31 paraffin-embedded cases of trophoblastic disease with flow cytometry. Twenty-four cases were histologically diagnosed as PM, 3 cases as CM; the others as hydropic abortion (2 cases), choriocarcinoma (1 case), and persistent trophoblastic disease (1 case). Four normal term placentas were used as diploidy controls. In 9 cases the results of the cytogenetic analysis were available. All placental specimens included also maternal tissue as an internal control. Eight of the 24 histologically diagnosed PMs were triploid; there was agreement in 8 cases out of 9 (90%) between the flow cytometric analysis and the karyotypic determination of ploidy. All normal controls as well as the hydropic abortion, the CM and the persistent trophoblastic disease were diploid. Abnormal content of DNA (DI = 1.3) was observed in the choriocarcinoma. Our results show that flow cytometric analysis of DNA content is a reliable and fast method of diagnosing PM on paraffin-embedded material. |
| Databáze: | OpenAIRE |
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