Tachykinin Antagonists Reverse Ischemia/Reperfusion Gastrointestinal Motility Impairment in Rats
| Autor: | Hanna Ługowska-Umer, Artur Umer, Krzysztof Kuziemski, Oliver Schönborn-Kellenberger, Paweł Konstanty Korolkiewicz, Łukasz Sein-Anand, R. Korolkiewicz |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Quinuclidines Carbachol Motility Pharmacology Saredutant 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Piperidines Tachykinins medicine.artery medicine Animals Humans Splanchnic Circulation Superior mesenteric artery Digestive System Surgical Procedures Receptors Tachykinin Gastrointestinal dysmotility Evans Blue Dose-Response Relationship Drug business.industry Rats Disease Models Animal chemistry Reperfusion Injury 030220 oncology & carcinogenesis Benzamides Quinolines 030211 gastroenterology & hepatology Surgery Gastrointestinal Motility Splanchnic Tachykinin receptor business medicine.drug |
| Zdroj: | Journal of Surgical Research. 255:510-516 |
| ISSN: | 0022-4804 |
| Popis: | Background Supraceliac aortic clamping and unclamping produces ischemia-reperfusion (I/R) injury of the splanchnic organs. The protective effects of tachykinin receptor antagonists, SR140333 (NK1 receptor), SR48968 (NK2 receptor), and SB222200 (NK3 receptor), against I/R-induced inhibition of intestinal motility were tested in rats. Material and methods The intestinal transit of Evans blue was measured in untreated rats and animals subjected to skin incision, I/R (1 h superior mesenteric artery occlusion followed by 24 h reperfusion) or sham operation. Surgical procedures were conducted under diethyl ether anesthesia. Results The gastrointestinal transit has not been markedly affected in rats, which were anesthetized or subjected to skin incision in comparison with untreated animals. In contrast, a sham operation and I/R have significantly reduced the intestinal motility. Pretreatment with NK1-3 blockers (SR140333 [3-30 μg/kg]; SR48968 [3-100 μg/kg]; and SB222200 [10-100 μg/kg]) reversed dose dependently the effects of I/R to the level observed after sham operation only. A combination of NK1+NK2+NK3 inhibitors exerted an additive effect compared with NK1 and NK2 antagonists used as single agents. Similarly, combined NK1+NK2 were more effective than NK2 alone. Sham operation and I/R have shifted the in vitro carbachol concentration–response curves to the right in comparison with untreated animals, a phenomenon partially reversed by NK1-NK3 pretreatment. Conclusions Single-agent and combined treatment with NK1-3 antagonists markedly attenuated the gastrointestinal dysmotility evoked by I/R injury. The pretreatment with NK3 blocker proved to be the most active in this experimental setting. |
| Databáze: | OpenAIRE |
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