Response of Congenitally Athymic (Nude) Mice to Infection With Mycobacterium boyis (Strain BCG)
| Autor: | James H. Vickers, Lynn E. Greenberg, Neal A. Sher, Sotiros D. Chaparas, E. Bruce Merchant |
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| Rok vydání: | 1975 |
| Předmět: |
Lung Diseases
Heterozygote Cancer Research Pathology medicine.medical_specialty Time Factors Lymphocyte Mice Nude Spleen Biology Monocytes Mice Phagocytosis medicine Animals Macrophage Lymphocytes Lung Mycobacterium Infections Granuloma Homozygote Histology Heterozygote advantage Organ Size T lymphocyte medicine.disease Mycobacterium bovis Molecular biology medicine.anatomical_structure Liver Oncology BCG Vaccine |
| Zdroj: | JNCI: Journal of the National Cancer Institute. 54:1419-1426 |
| ISSN: | 1460-2105 0027-8874 |
| DOI: | 10.1093/jnci/54.6.1419 |
| Popis: | Mice homozygous for the mutation "nude" (nu/nu) are athymic and lack thymus-dependent lymphocytes. Heterozygote (nu/+) controls, which are phenotypically and immunologically normal, and (nu/nu) mice were infected with 1.0 times 10-6 colony-forming units of Phipps strain BCG. In the spleens of nu/+ mice, there was a progressive increase in number of BCG up to the second week, followed by a gradual decline. However, in the nu/nu mice, BCG growth was gradual and continuous until termination of the experiment at 5 weeks. In the lung, significant differences were not noted until after the second week, at which time the nude mice showed a rapid increase (of more than 2 log10) in the number of BCG. However, the number of BCG was not significantly greater in the livers of either group. Changes in the normal histology of the lung included a massive influx of monocytes during the first 2 weeks which peaked at day 21. In the lungs of the nu/+ mice by day 28, there was considerable granuloma formation consisting of monocytes and small lymphocytes. However, in the lungs of nu/nu animals, the granulomas were made up primarily of monocytes with a lack of small lymphocytes. Acid-fast stains confirmed the presence of large numbers of organisms in the macrophages of nu/nu mice, with gradual destruction of these phagocytic cells. |
| Databáze: | OpenAIRE |
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