A fluorescent probe for hypochlorite with colorimetric and fluorometric characteristics and imaging in living cells
| Autor: | Cunjie Hu, Liqiang Yan, Jianping Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Biophysics Hypochlorite Hydroxylamine Photochemistry 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Coumarins Humans Fluorometry Molecular Biology Fluorescent Dyes 030304 developmental biology 0303 health sciences 010401 analytical chemistry Cell Biology Fluorescence Hypochlorous Acid 0104 chemical sciences Visual detection chemistry A549 Cells Color changes Colorimetry Naked eye |
| Zdroj: | Analytical Biochemistry. 566:32-36 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2018.11.011 |
| Popis: | An excellent fluorescent probe with “turn on” phenomenon for sensitive monitoring hypochlorite (ClO−) was prepared using the mild condensation reaction between coumarin and hydroxylamine (NH2OH). The probe possessed potent selectivity to ClO− with obvious color changes from yellow to light yellow and green to blue fluorescence emission, which could be noticed by the naked eye. Moreover, the probe has been succeeded in imaging ClO− in living A549 cells and thus has the potential prospect in the visual detection of intracellular ClO−. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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