CPEB1 coordinates alternative 3′-UTR formation with translational regulation
| Autor: | Felice-Alessio Bava, Juan Valcárcel, Carolina Eliscovich, Roderic Guigó, Raúl Méndez, Belén Miñana, Pedro G. Ferreira, Claudia Ben-Dov |
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| Rok vydání: | 2013 |
| Předmět: |
Untranslated region
Cytoplasm Polyadenylation Cytoplasmic polyadenylation element Cell Cycle Proteins Biology Translational regulation Splicing Factor U2AF Humans RNA Messenger Poly-ADP-Ribose Binding Proteins 3' Untranslated Regions Cell Proliferation Cell Nucleus mRNA Cleavage and Polyadenylation Factors Genetics Multidisciplinary Models Genetic Three prime untranslated region Alternative splicing Nuclear Proteins Cell biology Alternative Splicing Cell Transformation Neoplastic Ribonucleoproteins Protein Biosynthesis RNA splicing Poly A HeLa Cells Transcription Factors |
| Zdroj: | Nature. 495:121-125 |
| ISSN: | 1476-4687 0028-0836 |
| Popis: | More than half of mammalian genes generate multiple messenger RNA isoforms that differ in their 3' untranslated regions (3' UTRs) and therefore in regulatory sequences, often associated with cell proliferation and cancer; however, the mechanisms coordinating alternative 3'-UTR processing for specific mRNA populations remain poorly defined. Here we report that the cytoplasmic polyadenylation element binding protein 1 (CPEB1), an RNA-binding protein that regulates mRNA translation, also controls alternative 3'-UTR processing. CPEB1 shuttles to the nucleus, where it co-localizes with splicing factors and mediates shortening of hundreds of mRNA 3' UTRs, thereby modulating their translation efficiency in the cytoplasm. CPEB1-mediated 3'-UTR shortening correlates with cell proliferation and tumorigenesis. CPEB1 binding to pre-mRNAs not only directs the use of alternative polyadenylation sites, but also changes alternative splicing by preventing U2AF65 recruitment. Our results reveal a novel function of CPEB1 in mediating alternative 3'-UTR processing, which is coordinated with regulation of mRNA translation, through its dual nuclear and cytoplasmic functions. |
| Databáze: | OpenAIRE |
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