Biomimicry of oil infused layer on 3D printed poly(dimethylsiloxane): Non-fouling, antibacterial and promoting infected wound healing

Autor: Gaoxing Luo, Wen Zhong, Soroosh Derakhshanfar, Kaige Xu, Gaoqiang Shi, Ying Wang, Wu Jun, Malcolm Xing, Tengfei Liu, Yitian Wang
Rok vydání: 2017
Předmět:
Staphylococcus aureus
Materials science
Silver
Biocompatibility
Biofouling
Cell Survival
Bioengineering
02 engineering and technology
Microbial Sensitivity Tests
010402 general chemistry
01 natural sciences
Bacterial Adhesion
Nanocomposites
Biomaterials
chemistry.chemical_compound
Biomimetics
Spectroscopy
Fourier Transform Infrared
medicine
Escherichia coli
Animals
Dimethylpolysiloxanes
Cell Proliferation
Ions
Mice
Inbred BALB C
Wound Healing
Polydimethylsiloxane
Temperature
Granulation tissue
Fibroblasts
021001 nanoscience & nanotechnology
0104 chemical sciences
Anti-Bacterial Agents
Disease Models
Animal
Membrane
medicine.anatomical_structure
Chemical engineering
chemistry
Mechanics of Materials
Printing
Three-Dimensional
Granulation Tissue
Wound Infection
0210 nano-technology
Wound healing
Antibacterial activity
Rheology
Layer (electronics)
Oils
Zdroj: Materials scienceengineering. C, Materials for biological applications. 100
ISSN: 1873-0191
Popis: The nepenthes-inspired slippery liquid-infused surface has led to multiple potentials in biomedical devices' design. This study aims to develop a biomimetic, environmentally-friendly slippery layer of oil-infused 3D printed polydimethylsiloxane with anti-bacterial nanosilver (iPDMS/AgNPs) for wound dressing. The engineered 3D printed iPDMS can cater the different requirements of wounds with antifouling, anti-blood staining, and kill bacteria. iPDMS/AgNPs not only exhibits biocompatibility, against adherence and effective antibacterial activity but also effectively promotes neo-epithelial and granulation tissue formation to accelerate wound healing in vivo. Optimized rheologic parameters were obtained for the 3D printable iPDMS pre-polymerization condition. Scanning electronic micrograph (SEM) and Energy Dispersive Spectrometer (EDS) show a uniform mesh with AgNPs dotted on the printed dressing. No cytotoxicity of iPDMS/AgNPs has been found via cell Counting Kit-8(CCK-8) assay. Meanwhile, the membranes infused with silicon oil effectively prevented from the adherence of the two standard drug-resistant bacteria, Staphylococcus aureus and Escherichia coli (PDMS vs. PDMS+oil, p 0.05; PDMS+0.5%AgNPs vs. iPDMS+0.5%AgNPs, p 0.05; PDMS+2.5%AgNPs vs. iPDMS+2.5%AgNPs, p 0.05). By bacteria co-culture model iPDMS/AgNPs can kill about 80% of Staphylococcus aureus and Escherichia coli. When applied to a full-thickness wound defect model of murine, iPDMS/AgNPs was effective in anti-infection. It also promotes the epithelialization, the granulation tissue formation, and wound healing. These findings demonstrate that iPDMS/AgNPs may have therapeutic promise as an ideal wound dressing shortly.
Databáze: OpenAIRE
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