Expression patterns of connexin 26 and connexin 43 mRNA in canine benign and malignant mammary tumours
| Autor: | Tatsuya Ishida, Kayono Harada, Kazuhiko Yamada, Hozumi Yamamura, Kazuyuki Suzuki, Shizu Hashimoto, Haruka Gotoh, Ryuji Asano, Tsuneo Sato, Tomohiro Yano, Hiromi Hagiwara, Keiko Fukumoto |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Mammary gland
Connexin Mammary Neoplasms Animal Biology Malignancy Connexins law.invention Dogs law Gene expression otorhinolaryngologic diseases medicine Animals Dog Diseases RNA Messenger Gene Polymerase chain reaction Messenger RNA General Veterinary Reverse Transcriptase Polymerase Chain Reaction medicine.disease Molecular biology Connexin 26 Gene Expression Regulation Neoplastic Real-time polymerase chain reaction medicine.anatomical_structure Case-Control Studies Connexin 43 Female Animal Science and Zoology sense organs |
| Zdroj: | The Veterinary Journal. 172:178-180 |
| ISSN: | 1090-0233 |
| DOI: | 10.1016/j.tvjl.2005.02.023 |
| Popis: | The expression patterns of connexin (Cx) genes, encoding gap junctional proteins, are tissue- and cell-specific and, as their expression is mostly suppressed during carcinogenic processes, they are appropriate for monitoring tumour development. In this study, using reverse transcriptase-coupled polymerase chain reaction (RT-PCR), the expression of Cx mRNAs was examined in seven normal canine mammary glands and in 31 mammary gland tumour samples. Cx26 and Cx43 gene expression was studied in all normal tissues using specific Cx26 and Cx43 primers. When the expression patterns of Cx26 and Cx43 genes were analyzed in several types of canine mammary gland tumours, it was noted that it was the loss of Cx26 expression rather than the occurrence of Cx43 expression that was associated with malignancy. These results suggest that Cx26 plays an important role in tumourigenesis of canine mammary gland. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect