Mapping of fire blight resistance in Malus ×robusta 5 flowers following artificial inoculation
| Autor: | David Chagné, Thomas Wöhner, Magda-Viola Hanke, Erika Stefani, M. Troggio, Annette Wensing, Munazza Saeed, Andreas Peil, Henryk Flachowsky, Christine Hübert, Vincent G. M. Bus, Carolina Orellana-Torrejon, Klaus Richter, Ofere Francis Emeriewen, Susan E. Gardiner, M.B. Horner |
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| Rok vydání: | 2019 |
| Předmět: |
QTL mapping
0106 biological sciences 0301 basic medicine Malus Genetic Linkage Quantitative Trait Loci Population Flowers Plant Science Plant disease resistance Erwinia Genes Plant 01 natural sciences Malus ×robusta 5 Orchard inoculation 03 medical and health sciences lcsh:Botany Erwinia amylovora education Disease Resistance Plant Diseases education.field_of_study Bacterial disease biology Inoculation fungi food and beverages biology.organism_classification lcsh:QK1-989 Settore AGR/07 - GENETICA AGRARIA Horticulture 030104 developmental biology Shoot Fire blight Fire blight resistance Research Article 010606 plant biology & botany |
| Zdroj: | BMC Plant Biology, Vol 19, Iss 1, Pp 1-12 (2019) BMC Plant Biology |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/s12870-019-2154-7 |
| Popis: | Background Although the most common path of infection for fire blight, a severe bacterial disease on apple, is via host plant flowers, quantitative trait loci (QTLs) for fire blight resistance to date have exclusively been mapped following shoot inoculation. It is not known whether the same mechanism underlies flower and shoot resistance. Results We report the detection of a fire blight resistance QTL following independent artificial inoculation of flowers and shoots on two F1 segregating populations derived from crossing resistant Malus ×robusta 5 (Mr5) with susceptible ‘Idared’ and ‘Royal Gala’ in experimental orchards in Germany and New Zealand, respectively. QTL mapping of phenotypic datasets from artificial flower inoculation of the ‘Idared’ × Mr5 population with Erwinia amylovora over several years, and of the ‘Royal Gala’ × Mr5 population in a single year, revealed a single major QTL controlling floral fire blight resistance on linkage group 3 (LG3) of Mr5. This QTL corresponds to the QTL on LG3 reported previously for the ‘Idared’ × Mr5 and an ‘M9’ × Mr5 population following shoot inoculation in the glasshouse. Interval mapping of phenotypic data from shoot inoculations of subsets from both flower resistance populations re-confirmed that the resistance QTL is in the same position on LG3 of Mr5 as that for flower inoculation. These results provide strong evidence that fire blight resistance in Mr5 is controlled by a major QTL on LG3, independently of the mode of infection, rootstock and environment. Conclusions This study demonstrates for the first time that resistance to fire blight caused by Erwinia amylovora is independent of the mode of inoculation at least in Malus ×robusta 5. |
| Databáze: | OpenAIRE |
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