Label-free, live optical imaging of reprogrammed bipolar disorder patient-derived cells reveals a functional correlate of lithium responsiveness
| Autor: | S. M. Shamah, Alfred Xuyang Sun, Stephen J. Haggarty, Roy H. Perlis, J. L. Wang, Irwin D. Waldman |
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| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Bipolar Disorder Lithium (medication) Genome-wide association study Biology Cellular and Molecular Neuroscience chemistry.chemical_compound Lithium Carbonate medicine Humans Bipolar disorder Cell adhesion Biological Psychiatry Sodium-Bicarbonate Symporters Optical Imaging Lithium carbonate Cellular Reprogramming medicine.disease Phenotype 3. Good health Biomarker (cell) Affect Psychiatry and Mental health Treatment Outcome chemistry Pharmacogenetics Original Article Neuroscience Reprogramming Genome-Wide Association Study medicine.drug |
| Zdroj: | Translational Psychiatry |
| ISSN: | 2158-3188 |
| DOI: | 10.1038/tp.2014.72 |
| Popis: | Development of novel treatments and diagnostic tools for psychiatric illness has been hindered by the absence of cellular models of disease. With the advent of cellular reprogramming, it may be possible to recapitulate the disease biology of psychiatric disorders using patient skin cells transdifferentiated to neurons. However, efficiently identifying and characterizing relevant neuronal phenotypes in the absence of well-defined pathophysiology remains a challenge. In this study, we collected fibroblast samples from patients with bipolar 1 disorder, characterized by their lithium response (n=12), and healthy control subjects (n=6). We identified a cellular phenotype in reprogrammed neurons using a label-free imaging assay based on a nanostructured photonic crystal biosensor and found that an optical measure of cell adhesion was associated with clinical response to lithium treatment. This cellular phenotype may represent a useful biomarker to evaluate drug response and screen for novel therapeutics. |
| Databáze: | OpenAIRE |
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