Insecticidal activities of histone deacetylase inhibitors against a dipteran parasite of sheep, Lucilia cuprina
| Autor: | Neil H. Bagnall, Barney M. Hines, Praveer Gupta, Andrew J. Lucke, Robert Reid, Andrew C. Kotze, David P. Fairlie |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Insecticides Lucilia cuprina Biology Sesquiterpene lactone Histone Deacetylases Article Romidepsin lcsh:Infectious and parasitic diseases Insecticide Resistance 03 medical and health sciences chemistry.chemical_compound Inhibitory Concentration 50 0302 clinical medicine Depsipeptides Control medicine Animals Pharmacology (medical) Parthenolide lcsh:RC109-216 Histone deacetylase Sheep Domestic Insecticide Pharmacology Depsipeptide chemistry.chemical_classification Diptera Australia biology.organism_classification 3. Good health Histone Deacetylase Inhibitors 030104 developmental biology Infectious Diseases Enzyme Biochemistry chemistry 030220 oncology & carcinogenesis Larva Parasitology Apicidin medicine.drug |
| Zdroj: | International Journal for Parasitology: Drugs and Drug Resistance International Journal for Parasitology: Drugs and Drug Resistance, Vol 7, Iss 1, Pp 51-60 (2017) |
| ISSN: | 2211-3207 |
| Popis: | Histone deacetylase inhibitors (HDACi) are being investigated for the control of various human parasites. Here we investigate their potential as insecticides for the control of a major ecto-parasite of sheep, the Australian sheep blowfly, Lucilia cuprina. We assessed the ability of HDACi from various chemical classes to inhibit the development of blowfly larvae in vitro, and to inhibit HDAC activity in nuclear protein extracts prepared from blowfly eggs. The HDACi prodrug romidepsin, a cyclic depsipeptide that forms a thiolate, was the most potent inhibitor of larval growth, with equivalent or greater potency than three commercial blowfly insecticides. Other HDACi with potent activity were hydroxamic acids (trichostatin, CUDC-907, AR-42), a thioester (KD5170), a disulphide (Psammaplin A), and a cyclic tetrapeptide bearing a ketone (apicidin). On the other hand, no insecticidal activity was observed for certain other hydroxamic acids, fatty acids, and the sesquiterpene lactone parthenolide. The structural diversity of the 31 hydroxamic acids examined here revealed some structural requirements for insecticidal activity; for example, among compounds with flexible linear zinc-binding extensions, greater potency was observed in the presence of branched capping groups that likely make multiple interactions with the blowfly HDAC enzymes. The insecticidal activity correlated with inhibition of HDAC activity in blowfly nuclear protein extracts, indicating that the toxicity was most likely due to inhibition of HDAC enzymes in the blowfly larvae. The inhibitor potencies against blowfly larvae are different from inhibition of human HDACs, suggesting some selectivity for human over blowfly HDACs, and a potential for developing compounds with the inverse selectivity. In summary, these novel findings support blowfly HDAC enzymes as new targets for blowfly control, and point to development of HDAC inhibitors as a promising new class of insecticides. Graphical abstract Image 1 Highlights • We measured the insecticidal effects of histone deacetylase inhibitors against the sheep blowfly. • Insecticidal activity correlated with inhibition of HDAC enzyme activity in nuclear extracts. • Romidepsin showed equivalent or greater potency than commercial blowfly insecticides. • Some insights gained into structural requirements for insecticidal HDAC inhibitors. • Potential for HDAC inhibitors as insecticides. |
| Databáze: | OpenAIRE |
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