Screen-Printed Carbon Electrodes Modified by Rhodium Dioxide and Glucose Dehydrogenase
| Autor: | Karel Vytřas, Jan Soukup, Vojtěch Polan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Detection limit
Chromatography biology Article Subject Chemistry technology industry and agriculture chemistry.chemical_element macromolecular substances Biochemistry Rhodium lcsh:Biochemistry Linear range lcsh:Biology (General) Glucose dehydrogenase Electrode biology.protein Glucose oxidase lcsh:QD415-436 Molecular Biology Biosensor Carbon lcsh:QH301-705.5 Research Article |
| Zdroj: | Enzyme Research, Vol 2010 (2010) Enzyme Research |
| ISSN: | 2090-0414 |
| Popis: | The described glucose biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide, which functions as a mediator. The electrode is further modified by the enzyme glucose dehydrogenase, which is immobilized on the electrode's surface through electropolymerization with m-phenylenediamine. The enzyme biosensor was optimized and tested in model glucose samples. The biosensor showed a linear range of 500–5000 mg L−1 of glucose with a detection limit of 210 mg L−1 (established as 3σ) and response time of 39 s. When compared with similar glucose biosensors based on glucose oxidase, the main advantage is that neither ascorbic and uric acids nor paracetamol interfere measurements with this biosensor at selected potentials. |
| Databáze: | OpenAIRE |
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