Enhancement of the antiviral activity of poly r(A-U) by ametantrone and mitoxantrone
| Autor: | Keith Krabill, James M. Jamison, Daniel G. Flowers, Chun-che Tsai |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
musculoskeletal diseases
Ribonucleotide Ametantrone Pharmacology Vesicular stomatitis Indiana virus General Biochemistry Genetics and Molecular Biology Interferon medicine Humans General Pharmacology Toxicology and Pharmaceutics skin and connective tissue diseases Cells Cultured Mitoxantrone Molecular Structure Chemistry Drug Synergism General Medicine Double stranded rna Fibroblasts DNA Polymerase I Viral Inactivation humanities Poly C Poly A-U Biological Assay human activities medicine.drug |
| Zdroj: | Life Sciences. 46:653-661 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(90)90134-d |
| Popis: | The role of ametantrone (HAQ) and mitoxantrone (DHAQ) in modulating the antiviral and interferon-inducing activities of poly r(A-U) was examined using the humanforeskin fibroblast-vesicular stomatits virus (HSF-VSV) bioassay system in which the concentration of poly r(A-U) was fixed at 0.05 mM or 0.2 mM while the HAQ or DHAQ concentration was varied to produce variable HAQ (or DHAQ)/ribonucleotide ratios ranging from 1:16 to 2:1. HAQ, DHAQ and poly r(A-U) tested individually were not efficacious antiviral agents. When poly r(A-U) was combined with the ametantrone or mitoxantrone the antiviral activity was potentiated 10-fold at HAQ (or DHAQ)/ribonucleotide ratios in the region of 1 4 to 1 6 . The interferon-inducing activity of the HAQ (or DHAQ)/poly r(A-U) combinations were equal to the sum of the interferon-inducing activity of the poly r(A-U) and the HAQ (or DHAQ). These results indicate that the HAQ and DHAQ potentiate the antiviral activity of the poly r(A-U) without the superinduction of interferon. The direct viral inactivation study demonstrated that HAQ, DHAQ, poly r(A-U) and the HAQ (or DHAQ)/poly r(A-U) combinations did not inactivate the VSV at concentrations near the viral 50% inhibitory dose. |
| Databáze: | OpenAIRE |
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