Ethylene glycol and glycolic acid production by wild‐type Escherichia coli
| Autor: | Yao Yao, Zhongxi Zhang, Jian Hao, Xiyang Lu, Jiping Shi, Zorica Knezevic-Jugovic, Ljiljana Mojović, Yang Yang, Jinjie Gu, GJ Lye, Frank Baganz |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Ethylene Glycol Biomedical Engineering chemistry.chemical_element ethylene glycol Bioengineering xylonic acid Xylose medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Biosynthesis Aldehyde Reductase 010608 biotechnology Drug Discovery Escherichia coli medicine Glycolic acid 030304 developmental biology 0303 health sciences Escherichia coli Proteins Process Chemistry and Technology xylose technology industry and agriculture Substrate (chemistry) General Medicine Xylonic acid Aldehyde Oxidoreductases glycolic acid Glycolates coli chemistry Molecular Medicine Carbon Ethylene glycol Gene Deletion Biotechnology Nuclear chemistry |
| Zdroj: | Biotechnology and Applied Biochemistry |
| ISSN: | 1470-8744 0885-4513 |
| Popis: | Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization. |
| Databáze: | OpenAIRE |
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