Performance of bioMérieux Lowenstein–Jensen slopes in plastic tube packaging, compared to existing phenotypic methods, for efficient recovery of the Mycobacterium tuberculosis complex
| Autor: | Richard Gonzalez, Anthony de Cozar, Marissa Loubet, Remya Nambiar, Alex van Belkum, Camilla Rodrigues, Nabila Bereksi, Anjali Shetty |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Veterinary medicine Materials science 030106 microbiology Sensitivity and Specificity Microbiology 03 medical and health sciences Humans Tube (container) Tuberculosis Pulmonary Drug Packaging Plastic packaging Wide mouth Bacteriological Techniques biology Significant difference Sputum Becton dickinson Enhanced growth Mycobacterium tuberculosis General Medicine biology.organism_classification Culture Media 030104 developmental biology Mycobacterium tuberculosis complex Glass Bronchoalveolar Lavage Fluid Plastics |
| Zdroj: | Journal of Medical Microbiology. 68:398-401 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Purpose. Lowenstein–Jensen (LJ) medium used for cultivating Mycobacterium tuberculosis (MTB) is marketed in glass packaging. Breakage of glass slope is a major biosafety risk, especially during processing and storage, which gets magnified in large laboratories. We evaluated the performance of new bioMerieux (bMx) LJ slopes in plastic packaging, compared to bMx glass LJ medium and Becton Dickinson Mycobacterial Growth Indicator Tube (MGIT), for MTB recovery. Methodology. Consecutive pulmonary/extra-pulmonary samples (n=240) were processed using routine methods of decontamination, inoculation and incubation. Results. Plastic LJ slopes detected all 213 true-positive cases. The mean time-to-growth detection was 17.97 days for plastic LJ slopes, compared to 18.08 and 13.53 days for glass LJ slopes and MGIT, respectively. No statistically significant difference was observed between the two LJ slopes (P< 0.05). Both LJ slopes had a sensitivity and specificity of 100%, with respect to MGIT. Conclusion. Plastic LJ slopes are a good alternative to the traditional glass slopes. The medium quality did not differ with the packaging material. Increased surface area of these slants allowed enhanced growth, and the clear plastic material allowed accurate recording of growth. The wide mouth of these containers eased inoculation. Increased biosafety, by elimination of breakage risk, is the biggest advantage of this modification. |
| Databáze: | OpenAIRE |
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