Human umbilical cord blood-derived mesenchymal stem cells ameliorate psoriasis-like skin inflammation in mice
| Autor: | Tae-Yoon Kim, Kyung-Sun Kang, Yun Sang Lee, Ji Hyun Lee, Kwang-Won Seo, Shyam Kishor Sah |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chemokine T cell Biophysics Inflammation Anti-inflammatory effects Biochemistry Proinflammatory cytokine lcsh:Biochemistry 03 medical and health sciences Immune system Psoriasis medicine BMDC bone marrow-derived dendritic cell lcsh:QD415-436 lcsh:QH301-705.5 hUCB-MSC human umbilical cord blood-derived mesenchymal stem cell biology business.industry Mesenchymal stem cell Skin inflammation medicine.disease IL interleukin IDO indoleamine 2 3-dioxygenase 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Immunology biology.protein Mesenchymal stem cells CCL27 medicine.symptom business Research Article |
| Zdroj: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 9, Iss C, Pp 281-288 (2017) |
| ISSN: | 2405-5808 |
| Popis: | Mesenchymal stem cells (MSCs) inhibit the proliferation or activation of lymphocytes, and their inhibitory effects do not require human leukocyte antigen (HLA)-matching because MSCs express low levels of HLA molecules. Therefore, MSCs may be able to regulate immune responses. In this study, we determined whether MSCs could inhibit psoriasis-like skin inflammation in mice. After induction of psoriasis-like skin inflammation using intradermal injection of IL-23 or topical application of imiquimod with or without treatment with MSC, mouse skins were collected, and H&E staining and real-time PCR were performed. IL-23-induced skin inflammation was inhibited when MSCs were injected on day −1 and day 7. The expression of proinflammatory cytokines such as IL-6, IL-17, and TNF-α was inhibited by MSC injection, and the expression of chemokines such as CCL17, CCL20, and CCL27 was also decreased in mouse skin. We also determined whether MSCs could not only prevent but also treat psoriasis-like skin inflammation in mice. Furthermore, in vitro experiments also showed anti-inflammatory effects of MSCs. Dendritic cells which are co-cultured with MSCs suppressed CD4+ T cell activation and differentiation, which are important for the pathogenesis of psoriasis. These results suggest that MSCs could be useful for treating psoriasis. Highlights • Mesenchymal stem cells inhibit psoriasis-like skin inflammation in mice. • Mesenchymal stem cells modulate dendritic cell function. • Dendritic cells that co-cultured with mesenchymal stem cells regulate CD4+ T cell differentiation. |
| Databáze: | OpenAIRE |
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