Acid phosphatase. IV. Fluoride inhibition of prostatic acid phosphatase
| Autor: | John M. Reiner, K.K. Tsuboi, Perry B. Hudson |
|---|---|
| Rok vydání: | 1955 |
| Předmět: |
Male
chemistry.chemical_classification biology Acid Phosphatase Inorganic chemistry Prostate Biophysics Acid phosphatase Biochemistry Phosphoric Monoester Hydrolases Oxalate Phosphates Active center Fluorides chemistry.chemical_compound Enzyme Prostatic acid phosphatase chemistry Ionic strength biology.protein Humans Protein Tyrosine Phosphatases Molecular Biology Fluoride Organic anion |
| Zdroj: | Archives of Biochemistry and Biophysics. 56:165-183 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(55)90346-5 |
| Popis: | Inhibition of the acid phosphatase of human prostate by fluoride was shown to reach a maximum value at intermediate concentrations of fluoride and to be partially reversed by higher concentrations. The precise shape and location of the inhibition curve were influenced by ionic strength, by specific ions, and by protein concentration. A number of multivalent organic anions was shown to protect the enzyme against fluoride inhibition. The degree of protection afforded was greatest for anions such as citrate and oxalate. No evidence that this effect involves chelations of multivalent cations was found. A quantitative theory of these phenomena has been developed and shown to satisfy the experimental data, while some alternative theoretical possibilities have been eliminated or shown to be less plausible. The relation of these results to the structure of the active center of the enzyme is discussed. |
| Databáze: | OpenAIRE |
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