Optical biosensors based on Prussian Blue films
| Autor: | Anna Radomska, Robert Koncki, Tomasz Lenarczuk, Stanisław Głab |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Conductive polymer
Prussian blue Immobilized enzyme biology technology industry and agriculture macromolecular substances Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Chemical engineering Covalent bond Polymer chemistry Electrochemistry biology.protein Environmental Chemistry Molecule Glucose oxidase Hydrogen peroxide Biosensor Spectroscopy |
| Zdroj: | The Analyst. 126:1080-1085 |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | Optical biosensing schemes based on enzymatically modified inorganic/organic transparent films predominately composed of Prussian Blue are demonstrated. The composite film, which is non-electrochemically deposited on a non-conducting support, is used as an optical transducer for flow-through biosensors based on hydrolases and oxidases. Urease and glucose oxidase are utilized as model enzymes. Action of the urea biosensor is based on optical pH sensitivity of Prussian Blue indicator. The glucose biosensor is acting as first-generation optical biosensor based on in situ generated Prussian White transducer for hydrogen peroxide. These simple, single-pass transmission optical biosensors exhibit sensitivity in the millimolar range of concentration. The biosensors are very stable owing to presence of a poly(pyrrolylbenzoic acid) network in the composite material. This organic polymer plays a dual role as a binding agent for inorganic material and as a functionalized support for strong covalent immobilization of enzyme molecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|