A minimal actomyosin-based model predicts the dynamics of filopodia on neuronal dendrites
| Autor: | Tatyana Svitkina, Ji Yu, Nadia Efimova, Olena Marchenko, Charles W. Wolgemuth, Vladimir Rodionov, Sulagna Das, Leslie M. Loew, Igor L. Novak |
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| Rok vydání: | 2017 |
| Předmět: |
Models
Molecular 0301 basic medicine Neurogenesis Synaptogenesis Motility macromolecular substances Biology Hippocampus Microtubules Rats Sprague-Dawley 03 medical and health sciences Cell Movement Microtubule Myosin Animals Pseudopodia Molecular Biology Cells Cultured Actin Neurons Articles Actomyosin Dendrites Cell Biology Rats Dendritic filopodia Cell Motility Actin Cytoskeleton 030104 developmental biology Biophysics Filopodia |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e16-06-0461 |
| Popis: | A combination of computational and experimental approaches is used to show that the complex dynamics of dendritic filopodia, which is essential for synaptogenesis, is explained by a conceptually simple interplay among actin retrograde flow, myosin contractility, and substrate adhesion. Dendritic filopodia are actin-filled dynamic subcellular structures that sprout on neuronal dendrites during neurogenesis. The exploratory motion of the filopodia is crucial for synaptogenesis, but the underlying mechanisms are poorly understood. To study filopodial motility, we collected and analyzed image data on filopodia in cultured rat hippocampal neurons. We hypothesized that mechanical feedback among the actin retrograde flow, myosin activity, and substrate adhesion gives rise to various filopodial behaviors. We formulated a minimal one-dimensional partial differential equation model that reproduced the range of observed motility. To validate our model, we systematically manipulated experimental correlates of parameters in the model: substrate adhesion strength, actin polymerization rate, myosin contractility, and the integrity of the putative microtubule-based barrier at the filopodium base. The model predicts the response of the system to each of these experimental perturbations, supporting the hypothesis that our actomyosin-driven mechanism controls dendritic filopodia dynamics. |
| Databáze: | OpenAIRE |
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