Peripheral blood CD4+CRTH2+ cells in advanced stage non small cell lung cancer
| Autor: | Bülent Karagöz, Alpaslan Özgün, Orhan Türken, Ahmet Öztürk, Ahmet Alacacioglu, Özkan Sayan, Bahattin Yilmaz, Emin Gökhan Kandemir, Alev Akyol Erikçi, Oğuz Bilgi |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Pathology medicine.medical_treatment T cell Cell Gastroenterology survival Flow cytometry Blood cell crth2 Internal medicine medicine Cytotoxic T cell Lung cancer Chemotherapy medicine.diagnostic_test th2 cells business.industry General Medicine medicine.disease immunity lung cancer medicine.anatomical_structure Medicine business CD8 |
| Zdroj: | Open Medicine, Vol 5, Iss 4, Pp 431-436 (2010) |
| ISSN: | 2391-5463 |
| Popis: | To investigate CD4+CRTH2+ cells in peripheral blood in advanced stage non small cell lung cancer (NSCLC) patients. Forty-six patients with advanced stage NSCLC, who are chemotherapy or radiotherapy naïve, and 17 healthy volunteers, were enrolled in this study. The study was performed using flow cytometry and a complete blood cell counter analyser. CD4+ T cell percentage, CD4/CD8 ratio, CRTH2+CD4+ cell percentages, counts, and mean fluorescein intensity (MFI) and hematological parameters were evaluated in both groups. A survival analysis was performed to compare the patients with high CD4+CRTH2+ cell percentage and those with low CD4+CRTH2+ percentage. CD4+ T cell percentage in total lymphocytes and the CD4/CD8 ratio were lower in the patient group than in the control group. The absolute CD8 T cell count was higher in the patient group than in the control group, whereas the total T cells was not different. The CRTH2+ cell percentage in CD4+ T cells (7.96% ± 6.21% vs 3.37% ± 3.55%; respectively; p: 0,001) and the absolute count of CRTH2+CD4+ cells ( 97 mm-3 ± 109 mm-3 vs 37 mm-3 ± 38 mm-3, respectively; p: 0,033) in the patient group were higher than in the control group, but CRTH2-PE MFI values were not different between groups. Cox regression analysis did not show that CRTH2+CD4+ cell count or percentage is an independent prognostic factor. The study found that CRTH2 expression of CD4+ T cells and CRTH2+CD4+ cell number are higher in the peripheral blood of NSCLC patients than in that of healthy subjects. Further studies that explore the biological significance of high CD4+CRTH2+ cells in lung cancer patients, should be pursued. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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