Interaction of cells with patterned reactors
| Autor: | Chuntao Zhu, Xiaojun Han, Essi Taipaleenmäki, Brigitte Städler, Yan Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Cell Survival Biomedical Engineering 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Glucose Oxidase Bioreactors Coated Materials Biocompatible Cell Adhesion Human Umbilical Vein Endothelial Cells Humans General Materials Science Glucose oxidase Hydrogen peroxide chemistry.chemical_classification Liposome biology technology industry and agriculture Polymer Hep G2 Cells Hydrogen Peroxide equipment and supplies 021001 nanoscience & nanotechnology Enzymes Immobilized 0104 chemical sciences chemistry Chemical engineering Liposomes biology.protein Particle Nanoparticles 0210 nano-technology |
| Zdroj: | Zhu, C, Taipaleenmäki, E M, Zhang, Y, Han, X & Stadler, B M 2018, ' Interaction of cells with patterned reactors ', Biomaterials Science, vol. 6, no. 4, pp. 793-802 . https://doi.org/10.1039/C7BM00838D |
| ISSN: | 2047-4849 |
| DOI: | 10.1039/C7BM00838D |
| Popis: | Surface coatings that allow externally controlled interaction with cells are of interested for diverse biomedical applications. We fabricated particle patterns and assessed the interaction of these substrates with endothelial cells and hepatocytes. The particles were turned into subcompartmentalized reactors by immobilizing glucose oxidase loaded liposomes sandwiched between polymer layers. The reactor activities depending on the number of liposome deposition steps were confirmed in solution and on patterned surfaces. Finally, reduced viability of hepatocytes adhering to the reactor patterned surfaces in the presence of glucose was observed due to the local production of hydrogen peroxide. This first report on patterned reactors in combination with cells opens up vast opportunities to assemble interactive nanobiointerfaces. |
| Databáze: | OpenAIRE |
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